Western Blot Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate All lanes: IRAK4 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 38 kDa Observed band size: 55 kDa
Western Blot Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate All lanes: IRAK4 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 38 kDa Observed band size: 55 kDa
IRAK4 Recombinant Monoclonal Antibody
CSB-RA284992A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIRAK4
Overview
- SupplierCusabio
- Product NameIRAK4 Recombinant Monoclonal Antibody
- Delivery Days Customer20
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID10H4
- ConjugateUnconjugated
- Gene ID51135
- Target nameIRAK4
- Target descriptioninterleukin 1 receptor associated kinase 4
- Target synonymsIMD67; interleukin-1 receptor-associated kinase 4; IPD1; IRAK-4; NY-REN-64; REN64; renal carcinoma antigen NY-REN-64
- IsotypeIgG
- Scientific DescriptionSerine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203