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IRF2BP1 Antibody (aa109-122)

LS-C139734
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIRF2BP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IRF2BP1 Antibody (aa109-122)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell line HepG2 (calculated MW of 61.7kD according to NP_056464.1). Recommended concentration: 0.1-0.3 ug/ml.. Peptide-ELISA (1:32000), WB (0.1 - 0.3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell line HepG2 (calculated MW of 61.7kD according to NP_056464.1). Recommended concentration: 0.1-0.3 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26145
  • Target name
    IRF2BP1
  • Target description
    interferon regulatory factor 2 binding protein 1
  • Target synonyms
    interferon regulatory factor 2-binding protein 1; IRF-2-binding protein 1; probable E3 ubiquitin-protein ligase IRF2BP1; probable RING-type E3 ubiquitin transferase IRF2BP1
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRF2BP1 using anti-IRF2BP1 antibody (A11406-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF2BP1 antigen affinity purified polyclonal antibody (Catalog # A11406-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF2BP1 at approximately 72 kDa. The expected band size for IRF2BP1 is at 62 kDa.
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