Western blot analysis of extracts of various cell lines, using ISG15 antibody (TA377587) at 1:1000 dilution.Jurkat cells were treated by IFN-alpha (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using ISG15 antibody (TA377587) at 1:1000 dilution.Jurkat cells were treated by IFN-alpha (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.
ISG15 Rabbit Polyclonal Antibody
TA377587
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetISG15
Overview
- SupplierOriGene
- Product NameISG15 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID9636
- Target nameISG15
- Target descriptionISG15 ubiquitin like modifier
- Target synonymsG1P2; hUCRP; IFI15; IMD38; interferon, alpha-inducible protein (clone IFI-15K); interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; IP17; ubiquitin cross-reactive protein; ubiquitin-like protein ISG15; UCRP
- HostRabbit
- IsotypeIgG
- Scientific DescriptionISG15 Rabbit polyclonal Antibody
- ReactivityHuman, Rat
- UNSPSC12352203