Bio-Connect

ITGA4 / VLA-4 / CD49d Antibody (clone 9F10, AF647)

LS-C812041
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetITGA4
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGA4 / VLA-4 / CD49d Antibody (clone 9F10, AF647)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 microL of antibody per test (million cells in 100 microl staining volume or per 100 microl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9F10
  • Conjugate
    AlexaFluor 647
  • Estimated Purity
    ...
  • Gene ID3676
  • Target name
    ITGA4
  • Target description
    integrin subunit alpha 4
  • Target synonyms
    269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49D; IA4; integrin alpha-4; integrin alpha-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetITGA4
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsWestern Blot
TargetITGA4
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetITGA4
  • SizePrice
Western Blot analysis of K562 cells using Integrin alpha4 Polyclonal Antibody
Product group Antibodies
Cusabio
ITGA4 AntibodyCSB-PA005224
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetITGA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetITGA4
  • SizePrice
Product group Antibodies
Biosynth
CD49d antibody10R-CD49DAHU
  • SizePrice
Human U937 cells probed with Integrin alpha 4/CD49d Antibody, Unconjugated (bs-0641R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Product group Antibodies
References
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetITGA4
  • SizePrice
Western blot analysis in human cell line MOLT-4.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot
ReactivityHuman
TargetITGA4
  • SizePrice
Figure 1. Western blot analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody (PB9648). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: JURKAT Whole Cell Lysate at 40ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug, Lane 5: HEPG2 Whole Cell Lysate at 40ug, Lane 6: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 4 antigen affinity purified polyclonal antibody (Catalog # PB9648) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha 4 at approximately 220 kDa. The expected band size for Integrin alpha 4 is at 115 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetITGA4
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetITGA4
  • SizePrice