ITGAV/Integrin Alpha V/CD51 Antibody (C-Terminus)

LifeSpan BioSciences

ITGAV/Integrin Alpha V/CD51 Antibody (C-Terminus)

23

Recognizes CD51 (Integrin alpha V). The epitope recognized is on the cytoplasmic domain of the protein and it is thus intracellular. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 4 or alpha 6 integrin subunits. Species cross-reactivity: Human, g

Suitable for use in ELISA, RIA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 1:500. RIA: 1:500. Western Blot: 1:250. Recognizes a single band of 150kD when denatured, but not reduced. Under reducing conditions recognizes two major bands of 25 and 27kD, and to a lesser extent the full length 150kD band. This pattern is consistent with the fact that mature alpha V is composed of a disulfide bonded heavy (125kD) and a light (25kD) chains derived from a proteolytically cleaved precursor. Immunoprecipitation: 10 ul of antibody is sufficient to precipitate alpha V/beta 1 from 5x1E6 cells. Immunohistochemistry: 1:500. Acetone fixed tissue only.. ELISA (1:500), IHC, IHC-Fr (1:500), IP, RIA (1:500), WB (1:250) Suitable for use in ELISA, RIA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 1:500. RIA: 1:500. Western Blot: 1:250. Recognizes a single band of 150kD when denatured, but not reduced. Under reducing conditions recognizes two major bands of 25 and 27kD, and to a lesser extent the full length 150kD band. This pattern is consistent with the fact that mature alpha V is composed of a disulfide bonded heavy (125kD) and a light (25kD) chains derived from a proteolytically cleaved precursor. Immunoprecipitation: 10 ul of antibody is sufficient to precipitate alpha V/beta 1 from 5x1E6 cells. Immunohistochemistry: 1:500. Acetone fixed tissue only.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, RadioImmunoAssay

Research Use Only

Polyclonal

Unconjugated

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ITGAV

integrin subunit alpha V

antigen identified by monoclonal antibody L230; CD51; integrin alpha-V; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; vitronectin receptor subunit alpha; VNRA; VTNR

Rabbit

IgG

Equine, Goat, Human, Mouse, Porcine, Sheep

-20°C,2°C to 8°C

12352203