NS5 (JEV) antibody detects NS5 (JEV) protein by Western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NS5 (JEV) antibody (GTX131359) at a dilution of 1:3000.
NS5 (JEV) antibody detects NS5 (JEV) protein by Western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NS5 (JEV) antibody (GTX131359) at a dilution of 1:3000.
Japanese encephalitis virus NS5 antibody
GTX131359
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameJapanese encephalitis virus NS5 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.11 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThe small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. Non-structural protein 2B is a required cofactor for the serine protease function of NS3. Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2-O positions.
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Li C, Zhou Y, Chen X, et al. Porcine TRIM35 positively regulate TRAF3-mediated IFN-β production and inhibit Japanese encephalitis virus replication. Dev Comp Immunol. 2022,127:104290. doi: 10.1016/j.dci.2021.104290Read this paper
- Yu D, Zhao Y, Pan J, et al. C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein. Virol Sin. 2021,36(6):1443-1455. doi: 10.1007/s12250-021-00423-6Read this paper
- Sehrawat S, Khasa R, Deb A, et al. Valosin-containing protein/p97 plays critical roles in the Japanese encephalitis virus life cycle. J Virol. 2021,95(11):pii: JVI.02336-20. doi: 10.1128/JVI.02336-20.Read this paper
- Li C, Di D, Huang H, et al. NS5-V372A and NS5-H386Y variations are responsible for differences in interferon α/β induction and co-contribute to the replication advantage of Japanese encephalitis virus genotype I over genotype III in ducklings. PLoS Pathog. 2020,16(9):e1008773. doi: 10.1371/journal.ppat.1008773Read this paper
- Carr M, Gonzalez G, Martinelli A, et al. Upregulated expression of the antioxidant sestrin 2 identified by transcriptomic analysis of Japanese encephalitis virus-infected SH-SY5Y neuroblastoma cells. Virus Genes. 2019,55(5):630-642. doi: 10.1007/s11262-019-01683-xRead this paper