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ProductsBack/JUNB / JUN-B Antibody (clone 204C4a)

JUNB / JUN-B Antibody (clone 204C4a)

LS-C342547
LifeSpan BioSciences
ApplicationsDot Blot, Western Blot
Product group Antibodies
TargetJUNB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    JUNB / JUN-B Antibody (clone 204C4a)
  • Delivery Days Customer
    23
  • Applications
    Dot Blot, Western Blot
  • Applications Supplier
    DB, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    204C4a
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3726
  • Target name
    JUNB
  • Target description
    JunB proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    activator protein 1; AP-1; jun B proto-oncogene; transcription factor jun-B
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JUNB using anti-JUNB antibody (A01825-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUNB antigen affinity purified polyclonal antibody (Catalog # A01825-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUNB at approximately 43-45KD. The expected band size for JUNB is at 36KD.
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