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ProductsBack/KPNA1 / Importin Alpha 5 Antibody (clone 114-E12)

KPNA1 / Importin Alpha 5 Antibody (clone 114-E12)

LS-C16999
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetKPNA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    KPNA1 / Importin Alpha 5 Antibody (clone 114-E12)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Immunoprecipitation, Immunofluorescence, ELISA and Western Blot. Immunoprecipitation (native): 5-10 ug. Immunofluorescence: 5-10 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.. ELISA (0.1 - 1 µg/ml), IF (5 - 10 µg/ml), IP, WB (1 - 3 µg/ml) Suitable for use in Immunoprecipitation, Immunofluorescence, ELISA and Western Blot. Immunoprecipitation (native): 5-10 ug. Immunofluorescence: 5-10 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    114-E12
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3836
  • Target name
    KPNA1
  • Target description
    karyopherin subunit alpha 1
  • Target synonyms
    importin alpha 5; importin subunit alpha-1; importin subunit alpha-5; importin-alpha-S1; IPOA5; karyopherin alpha 1 (importin alpha 5); NPI-1; nucleoprotein interactor 1; RAG cohort protein 2; RCH2; recombination activating gene cohort 2; SRP1; SRP1-beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SRP1/KPNA1 using anti-SRP1/KPNA1 antibody (A04024-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRP1/KPNA1 antigen affinity purified polyclonal antibody (Catalog # A04024-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRP1/KPNA1 at approximately 60KD. The expected band size for SRP1/KPNA1 is at 60KD.
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