R-17F is reported to exhibit potent dose-dependent cytotoxicity when it is added to living human iPS/ES cells. [Left] After the incubation of iPS cell suspension with R-17F at 4oC for only 45 minutes, the percentage of viable cells decreased concentration-dependently (red triangles). Blue squares : effects of the isotype (IgG1)-matching control antibody (anti-alpha-MBP) as Negative Control. [Right] When R-17F-treated iPS cells were incubated with a small amount (0.025-0.1 microg) of the secondary antibody (goat anti-mouse IgG1 antibody), the cytotoxic effect of R-17F was enhanced significantly in a dose-dependent manner (red triangles).
![lacto-N-fucopentaose I antibody [R-17F]](https://www.genetex.com/upload/website/prouct_img/normal/GTX57213/GTX57213_GTX57213_table-1_w_23061123_651.webp "lacto-N-fucopentaose I antibody [R-17F]")
![Cultured human iPS cells were stained with R-17F, SSEA-3, and SSEA-4 antibodies. [bars: 100microm] R-17F stained the entire surface of the cell membranes equally, while the staining by SSEA-3 and SSEA-4 antibodies are not evenly. This suggests that R-17F epitope is expressed ubiquitously all over the human iPS cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX57213/GTX57213_ICC_IF_1-1_w_23061123_702.webp "Cultured human iPS cells were stained with R-17F, SSEA-3, and SSEA-4 antibodies. [bars: 100microm] R-17F stained the entire surface of the cell membranes equally, while the staining by SSEA-3 and SSEA-4 antibodies are not evenly. This suggests that R-17F epitope is expressed ubiquitously all over the human iPS cells.")
![WB analysis of human iPS and HEK293 cell lysate using GTX57213 lacto-N-fucopentaose I antibody [R-17F]. One major positive band and several minor bands were specific to human iPS cells, and any positive band was not obtained with HEK293 cells. Human iPS cell: LNFP I positive HEK293 cell lysate: Negative Control Loading : 5ug per lane Dilution : 1:2000](https://www.genetex.com/upload/website/prouct_img/normal/GTX57213/GTX57213_WB_1_w_23061123_486.webp "WB analysis of human iPS and HEK293 cell lysate using GTX57213 lacto-N-fucopentaose I antibody [R-17F]. One major positive band and several minor bands were specific to human iPS cells, and any positive band was not obtained with HEK293 cells. Human iPS cell: LNFP I positive HEK293 cell lysate: Negative Control Loading : 5ug per lane Dilution : 1:2000")
R-17F is reported to exhibit potent dose-dependent cytotoxicity when it is added to living human iPS/ES cells. [Left] After the incubation of iPS cell suspension with R-17F at 4oC for only 45 minutes, the percentage of viable cells decreased concentration-dependently (red triangles). Blue squares : effects of the isotype (IgG1)-matching control antibody (anti-alpha-MBP) as Negative Control. [Right] When R-17F-treated iPS cells were incubated with a small amount (0.025-0.1 microg) of the secondary antibody (goat anti-mouse IgG1 antibody), the cytotoxic effect of R-17F was enhanced significantly in a dose-dependent manner (red triangles).
lacto-N-fucopentaose I antibody [R-17F]
GTX57213
ApplicationsFunctional Assay, Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
Overview
- SupplierGeneTex
- Product Namelacto-N-fucopentaose I antibody [R-17F]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes lacto-N-fucopentaose I (LNFP I: Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc) on a glycolipid / glycoprotein. R-17F epitopes are expressed on undifferentiated human induced pluripotent stem (iPS) / embryonic stem (ES) ce
- Application Supplier NoteWB: 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFunctional Assay, Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDR-17F
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203