lambdaDNA/ HindIII Digest

Biorbyt

lambdaDNA/ HindIII Digest

16

Preparation: Lambda DNA (48.502 bp) was completely digested by HindIII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Recommended Load: 5 microl per lane.

Research Use Only

100 ng/ul

The HindIII digest of lambda DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125. The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min.

-20°C

12352204