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ProductsBack/LAT2 / NTAL Antibody (aa91-243, clone NAP-07, PE)

LAT2 / NTAL Antibody (aa91-243, clone NAP-07, PE)

LS-C46507
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetLAT2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LAT2 / NTAL Antibody (aa91-243, clone NAP-07, PE)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suggested working dilution is 1:30. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    Flo (1:30), ICC, IP, WB Suggested working dilution is 1:30. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    NAP-07
  • Concentration
    0.1 mg/ml
  • Conjugate
    RPE
  • Estimated Purity
    ...
  • Gene ID7462
  • Target name
    LAT2
  • Target description
    linker for activation of T cells family member 2
  • Target synonyms
    HSPC046; LAB; linker for activation of B-cells; linker for activation of T cells, transmembrane adaptor 2; linker for activation of T-cells family member 2; membrane-associated adapter molecule; non-T-cell activation linker; NTAL; WBSCR15; WBSCR5; Williams-Beuren syndrome chromosomal region 15 protein; Williams-Beuren syndrome chromosomal region 5 protein; WSCR5
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NTAL/LAT2 using anti-NTAL/LAT2 antibody (A02496-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTAL/LAT2 antigen affinity purified polyclonal antibody (Catalog # A02496-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTAL/LAT2 at approximately 27 kDa. The expected band size for NTAL/LAT2 is at 27 kDa.
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