LDB1 antibody

Biorbyt

LDB1 antibody

16

This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene; a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20microg for 10E8 cells for ChIP.

Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA

ELISA: 1:425,000, ChIP: User Optimized, WB: 1:500 - 1:3,000 ChIP, ELISA, WB

Research Use Only

Polyclonal

1.80 mg/ml

Unconjugated

100%

Liquid

Ldb1

LIM domain binding 1

carboxyl-terminal LIM domain-binding protein 2; CLI; CLIM2; LIM domain-binding factor CLIM2; LIM domain-binding protein 1; NLI; nuclear LIM interactor

Rabbit

IgG

LIM domain-binding protein 1

LDB1 antibody

Human

-20°C,2°C to 8°C

12352203