LDOC1 Antibody (N-Terminus) LS-B3527

Biorbyt

LDOC1 Antibody (N-Terminus) LS-B3527

10

Further information: Immunohistochemistry: LS-B3527 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3527 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml) IHC, IHC-P, WB

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

Rabbit

IgG

LDOC1 Antibody (N-Terminus) LS-B3527

Human

-20°C,2°C to 8°C

12352203