ProductsBack/LEO1 Antibody (phospho-Ser10, PE)

LEO1 Antibody (phospho-Ser10, PE)

LS-C206702

ApplicationsDot Blot, ELISA

Product group Antibodies

TargetLEO1

Overview

Supplier
LifeSpan BioSciences
Product Name
LEO1 Antibody (phospho-Ser10, PE)
Delivery Days Customer
23
Application Supplier Note
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Applications
Dot Blot, ELISA
Applications Supplier
DB, ELISA The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
RPE
Estimated Purity
...
Gene ID123169
Target name
LEO1
Target description
LEO1 homolog, Paf1/RNA polymerase II complex component
Target synonyms
Leo1 Paf1/RNA polymerase II complex component; Leo1, Paf1/RNA polymerase II complex component, homolog; RDL; replicative senescence down-regulated leo1-like protein; RNA polymerase-associated protein LEO1
Host
Rabbit
Isotype
IgG
Storage Instruction
-20°C,2°C to 8°C
UNSPSC
12352203

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Figure 1. Western blot analysis of LEO1 using anti-LEO1 antibody (A06433-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEO1 antigen affinity purified polyclonal antibody (Catalog # A06433-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LEO1 at approximately 105 kDa. The expected band size for LEO1 is at 75 kDa.

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