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ProductsBack/LF / LTF / Lactoferrin Antibody (Biotin)

LF / LTF / Lactoferrin Antibody (Biotin)

LS-C185111
LifeSpan BioSciences
ApplicationsImmunoElectrophoresis, ELISA
Product group Antibodies
TargetLTF
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LF / LTF / Lactoferrin Antibody (Biotin)
  • Delivery Days Customer
    14
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ImmunoElectrophoresis, ELISA
  • Applications Supplier
    ELISA (1:1000 - 1:7000), IE The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID4057
  • Target name
    LTF
  • Target description
    lactotransferrin
  • Target synonyms
    epididymis luminal protein 110; GIG12; growth-inhibiting protein 12; HEL110; HLF2; kaliocin-1; lactoferricin; lactoferroxin; lactotransferrin; LF; neutrophil lactoferrin; talalactoferrin
  • Host
    Goat
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lactoferrin using anti-Lactoferrin antibody (A00633-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lactoferrin antigen affinity purified polyclonal antibody (Catalog # A00633-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lactoferrin at approximately 85 kDa. The expected band size for Lactoferrin is at 78 kDa.
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