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ProductsBack/LIG3 / DNA Ligase III Antibody (aa765-1009)

LIG3 / DNA Ligase III Antibody (aa765-1009)

LS-C185529
LifeSpan BioSciences
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLIG3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LIG3 / DNA Ligase III Antibody (aa765-1009)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000) IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.04 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3980
  • Target name
    LIG3
  • Target description
    DNA ligase 3
  • Target synonyms
    DNA ligase 3; LIG2; LIG3alpha; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DNA Ligase III/LIG3 using anti-DNA Ligase III/LIG3 antibody (A02741-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Ligase III/LIG3 antigen affinity purified polyclonal antibody (Catalog # A02741-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Ligase III/LIG3 at approximately 113 kDa. The expected band size for DNA Ligase III/LIG3 is at 113 kDa.
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