LIN28B Antibody

Cusabio

LIN28B Antibody

20

Western Blot, ELISA

Research Use Only

Polyclonal

Unconjugated

Rabbit

IgG

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5-GGAG-3 motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5-GGAG-3 motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma. Guo Y., Chen Y., Ito H., et al.Gene 384:51-61(2006) Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. Heo I., Joo C., Cho J., Ha M., Han J., Kim V.N. Mol. Cell 32:276-284(2008) TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation. Heo I., Joo C., Kim Y.-K., Kim V.N.,et al. Cell 138:696-708(2009)

Human

-20°C or -80°C

12352203