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Lum Antibody

CSB-PA013234ZA01MO
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLum
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Overview

  • Supplier
    Cusabio
  • Product Name
    Lum Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID17022
  • Target name
    Lum
  • Target description
    lumican
  • Target synonyms
    keratan sulfate proteoglycan lumican; KSPG lumican; Ld; Ldc; lumican; SL; SLRR2D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51885
  • Protein Name
    Lumican
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lumican using anti-Lumican antibody (PB9662). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Testis Tissue Lysate at 50ug, Lane 2: Mouse Thymus Tissue Lysate at 50ug, Lane 3: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lumican antigen affinity purified polyclonal antibody (Catalog # PB9662) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lumican at approximately 60 kDa. The expected band size for Lumican is at 38 kDa.
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