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Description
Catalog number
CAS No.
Clone ID
News
Vorige
Volgende
Human AKT1 / PKB / PKBalpha Baculovirus-Insect Cells Transfected Lysate (positive control) (denatured)
Catalog number:
10763-H08BL-300
Brand:
Sino Biological
Packing:
300 ug
Price:
On Request
Expected delivery time:
10 days
Quantity:
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General
Product specifications for - Human AKT1 / PKB / PKBalpha Baculovirus-Insect Cells Transfected Lysate (positive control) (denatured)
Overview:
Product group:
Lysates / Extracts
Category:
Lysates
Application:
Western Blot
Application note:
WB: Use at an assay dependent dilution.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Target:
AKT1
Target description:
v-akt murine thymoma viral oncogene homolog 1
Properties:
Datasheet:
Datasheet
Research Use Only
UNSPSC:
41105330
Storage instructions:
Store at -80-¦C. Aliquot to avoid repeated freezing and thawing
Scientific information:
Scientific info:
Baculovirus-Insect Cells transfected lysate in which Human AKT1 / PKB / PKBalpha has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer. Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized; Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Gene ID:
207
Additional information:
Synonyms:
10763-H08BL; 10763-H08BL-300; AKT1; RAC-ALPHA; RAC; PRKBA; PKB-ALPHA; PKB; CWS6; AKT; rac protein kinase alpha; proto-oncogene c-Akt; protein kinase B alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; PKB alpha; v-akt murine thymoma viral oncogene homolog 1