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Human AKT1 / PKB / PKBalpha Baculovirus-Insect Cells Transfected Lysate (positive control) (denatured)

Catalog number: 10763-H08BL-300
Brand: Sino Biological
Packing: 300 ug
Price: On Request
Expected delivery time: 10 days
Quantity:
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Product specifications for - Human AKT1 / PKB / PKBalpha Baculovirus-Insect Cells Transfected Lysate (positive control) (denatured)

Overview: 
Product group: Lysates / Extracts
Category: Lysates
Application: Western Blot
Application note: WB: Use at an assay dependent dilution.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Target: AKT1
Target description: v-akt murine thymoma viral oncogene homolog 1
Properties: 
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41105330
Storage instructions: Store at -80-¦C. Aliquot to avoid repeated freezing and thawing
Scientific information: 
Scientific info: Baculovirus-Insect Cells transfected lysate in which Human AKT1 / PKB / PKBalpha has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer. Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized; Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Gene ID: 207
Additional information: 
Synonyms: 10763-H08BL; 10763-H08BL-300; AKT1; RAC-ALPHA; RAC; PRKBA; PKB-ALPHA; PKB; CWS6; AKT; rac protein kinase alpha; proto-oncogene c-Akt; protein kinase B alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; PKB alpha; v-akt murine thymoma viral oncogene homolog 1