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Western blot analysis of extracts of various cell lines using LYZ Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using LYZ Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using LYZ Polyclonal Antibody at dilution of 1:3000.

Lysozyme Polyclonal Antibody

E-AB-64686
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    Lysozyme Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lyz1/2 using anti-Lyz1/2 antibody (A32724). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lyz1/2 antigen affinity purified polyclonal antibody (Catalog # A32724) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lyz1/2 at approximately 14 kDa. The expected band size for Lyz1/2 is at 17 kDa.
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