IHC-P analysis of human kidney tissue using GTX22805 mAChR M2 antibody [31-1D1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![ICC/IF analysis of SH-SY5Y cells using GTX22805 mAChR M2 antibody [31-1D1]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22805/GTX22805_479_ICC-IF_w_23060620_214.webp "ICC/IF analysis of SH-SY5Y cells using GTX22805 mAChR M2 antibody [31-1D1]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
IHC-P analysis of human kidney tissue using GTX22805 mAChR M2 antibody [31-1D1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
mAChR M2 antibody [31-1D1]
GTX22805
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Rat
TargetCHRM2
Overview
- SupplierGeneTex
- Product NamemAChR M2 antibody [31-1D1]
- Delivery Days Customer9
- Antibody SpecificityThis antibody is specific for the m2 mAChR subtype.
- Application Supplier NoteWB: 1:1,000. ICC/IF: 2microg/ml. IHC-P: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID31-1D1
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID397498
- Target nameCHRM2
- Target descriptioncholinergic receptor muscarinic 2
- Target synonymscardiac muscarinic acetylcholine receptor; muscarinic acetylcholine receptor M2
- HostMouse
- IsotypeIgG1
- Protein IDP06199
- Protein NameMuscarinic acetylcholine receptor M2
- ReactivityHuman, Mouse, Porcine, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Single onabotulinumtoxinA 200U dose improved clinical symptoms but not urothelial dysfunction in neurogenic detrusor overactivity due to spinal cord injury. Chen SF et al., 2019 Jan, J Formos Med AssocRead more
- Urothelial Barrier Deficits, Suburothelial Inflammation and Altered Sensory Protein Expression in Detrusor Underactivity. Jiang YH et al., 2017 Jan, J UrolRead more