Sample (30 μg of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX109519 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![MAD1 antibody detects MAD1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody (GTX109519) diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] (GTX70268) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109519/GTX109519_40030_20150410_IFA_w_23060500_496.webp "MAD1 antibody detects MAD1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody (GTX109519) diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] (GTX70268) diluted at 1:500. Blue: Hoechst 33342 staining.")
Sample (30 μg of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX109519 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MAD1 antibody
GTX109519
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mammals, Mouse, Rat
TargetMAD1L1
Overview
- SupplierGeneTex
- Product NameMAD1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8379
- Target nameMAD1L1
- Target descriptionmitotic arrest deficient 1 like 1
- Target synonymsMAD1, MVA7, PIG9, TP53I9, TXBP181, mitotic spindle assembly checkpoint protein MAD1, MAD1 mitotic arrest deficient like 1, MAD1-like protein 1, mitotic arrest deficient 1-like protein 1, mitotic checkpoint MAD1 protein homolog, mitotic-arrest deficient 1, yeast, homolog-like 1, tax-binding protein 181, tumor protein p53 inducible protein 9
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y6D9
- Protein NameMitotic spindle assembly checkpoint protein MAD1
- Scientific DescriptionMAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mammals, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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