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MAFG antibody

GTX114541
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMAFG
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAFG antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4097
  • Target name
    MAFG
  • Target description
    MAF bZIP transcription factor G
  • Target synonyms
    basic leucine zipper transcription factor MafG; hMAF; transcription factor MafG; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15525
  • Protein Name
    Transcription factor MafG
  • Scientific Description
    Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant.
    Read more
  • YAP Inactivation by Soft Mechanotransduction Relieves MAFG for Tumor Cell Dedifferentiation.
    Read more
  • Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations.
    Read more
  • MAFG-driven astrocytes promote CNS inflammation. Wheeler MA et al., 2020 Feb, Nature
    Read more
  • A MAFG-lncRNA axis links systemic nutrient abundance to hepatic glucose metabolism. Pradas-Juni M et al., 2020 Jan 31, Nat Commun
    Read more
  • miR-128 Is Implicated in Stress Responses by Targeting MAFG in Skeletal Muscle Cells. Caggiano R et al., 2017, Oxid Med Cell Longev
    Read more
  • MAFG is a transcriptional repressor of bile acid synthesis and metabolism. de Aguiar Vallim TQ et al., 2015 Feb 3, Cell Metab
    Read more

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Figure 1. Western blot analysis of MAFG using anti-MAFG antibody (A04636-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAFG antigen affinity purified polyclonal antibody (Catalog # A04636-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAFG at approximately 18 kDa. The expected band size for MAFG is at 18 kDa.
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