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Mannose Binding Lectin (MBL) Polyclonal Antibody

CAU25853
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetMbl2
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Overview

  • Supplier
    Biomatik
  • Product Name
    Mannose Binding Lectin (MBL) Polycl
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64668
  • Target name
    Mbl2
  • Target description
    mannose binding lectin 2
  • Target synonyms
    Ab2-001; Ab2-011; mannan-binding protein; mannose binding lectin 2 (protein C); mannose-binding lectin (protein C) 2; mannose-binding protein C; mannose-binding protein C (liver); mannose-binding protein C-like; MBP-C; RA-reactive factor P28A subunit; ra-reactive factor polysaccharide-binding component p28A; raRF p28A; RARF/P28A
  • Host
    Rabbit
  • Protein IDP08661
  • Protein Name
    Mannose-binding protein C
  • Scientific Description
    The Mannose Binding Lectin (MBL) Polyclonal Antibody (Species: Rat) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26KD. The expected band size for MBL2 is at 26KD.
Product group Antibodies
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TargetMbl2
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