MAP1A antibody [HM-1]

GeneTex

MAP1A antibody [HM-1]

9

The antibody does not cross-react with other MAPs or tubulin. By immunohistochemical staining of brain tissue, the antibody shows selective labeling of neurons with stronger staining of axons than dendrites.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

HM-1

0.1 mg/ml

Unconjugated

Map1a

microtubule-associated protein 1A

MAP-1A; microtubule-associated protein 1A; Mtap1a

Mouse

IgG1

Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins (MAPs). There is considerable evidence that MAPs may mediate the binding of membranous organelles, actin filaments and intermediate filaments to microtubules, leading to the speculation that they may therefore be important for cellular processes such as mitosis and organelle transport, and for determining the dynamic properties of the cytoskeleton. Two classes of high molecular weight components termed MAP1 and MAP2 have been demonstrated to co-purify with tubulin during cycles of microtubule assembly and disassembly, and to stimulate microtubule assembly in vitro. MAP1 is one of the major neuronal MAPs as well as being the largest (350 kD). Purified preparations of MAP1 from bovine brain have been demonstrated to contain at least two low molecualr weight components (19-34 kD) that remain tightly associated with MAP1 heavy chains under nondenaturing conditions. In contrast to MAP2 which is localized primarily in the dendrites of neurons in brain and possibly in small amounts in other cells, MAP1 is more generally distributed, being found in both dendrites and axons of neurons and in glial cells in brain, in chromtophores, and on both interphase and mitotic microtubules in various tissue culture cells, suggesting that MAP1 may have a more general function. In the newborn rat brain the expression of MAP1 is almost absent. Its levels begin to increase from postnatal day 5 and increase steadily, in step with neuronal differentiation, reaching a maximum around postnatal day 28, the time when neurons have reached their mature morphology. MAP1 is degraded by a Cathepsin D like protease in the brain of aged rats. In developmental neurobiology MAP1 acts as a marker of neuronal maturation.

Mouse, Rat

2°C to 8°C,-20°C or -80°C

12352203