Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP3K8 (Ser400) Polyclonal Antibody, Unconjugated (bs-3454R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
MAP3K8 (Ser400) Polyclonal Antibody
BS-3454R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetMAP3K8
Overview
- SupplierBioss Antibodies
- Product NameMAP3K8 (Ser400) Polyclonal Antibody
- Delivery Days Customer16
- Antibody SpecificityThis phosphorylation site is homologous across the listed species.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1326
- Target nameMAP3K8
- Target descriptionmitogen-activated protein kinase kinase kinase 8
- Target synonymsaugmented in rheumatoid arthritis 2; AURA2; c-COT; COT; cot (cancer Osaka thyroid) oncogene; EST; ESTF; Ewing sarcoma transformant; MEKK8; mitogen-activated protein kinase kinase kinase 8; proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; TPL2; Tpl-2; tumor progression locus 2
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDP41279
- Protein NameMitogen-activated protein kinase kinase kinase 8
- ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203