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Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP3K8 (Ser400) Polyclonal Antibody, Unconjugated (bs-3454R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP3K8 (Ser400) Polyclonal Antibody, Unconjugated (bs-3454R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP3K8 (Ser400) Polyclonal Antibody, Unconjugated (bs-3454R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

MAP3K8 (Ser400) Polyclonal Antibody

Research Use Only
BS-3454R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetMAP3K8
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MAP3K8 (Ser400) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Antibody Specificity
    This phosphorylation site is homologous across the listed species.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1326
  • Target name
    MAP3K8
  • Target description
    mitogen-activated protein kinase kinase kinase 8
  • Target synonyms
    augmented in rheumatoid arthritis 2; AURA2; c-COT; COT; cot (cancer Osaka thyroid) oncogene; EST; ESTF; Ewing sarcoma transformant; MEKK8; mitogen-activated protein kinase kinase kinase 8; proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; TPL2; Tpl-2; tumor progression locus 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Modification Type
    Phosphorylated
  • Protein IDP41279
  • Protein Name
    Mitogen-activated protein kinase kinase kinase 8
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203