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ProductsBack/MARCHF2 Rabbit Polyclonal Antibody
Gel: 8%SDS-PAGE Lysate: 40 microg Lane: Human fetal liver tissue Primary antibody: TA365278 (MARCHF2 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds
Gel: 8%SDS-PAGE Lysate: 40 microg Lane: Human fetal liver tissue Primary antibody: TA365278 (MARCHF2 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds
Gel: 8%SDS-PAGE Lysate: 40 microg Lane: Human fetal liver tissue Primary antibody: TA365278 (MARCHF2 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

MARCHF2 Rabbit Polyclonal Antibody

TA365278
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetMARCHF2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MARCHF2 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID51257
  • Target name
    MARCHF2
  • Target description
    membrane associated ring-CH-type finger 2
  • Target synonyms
    E3 ubiquitin-protein ligase MARCH2; E3 ubiquitin-protein ligase MARCHF2; HSPC240; MARCH2; MARCH-II; membrane associated ring finger 2; membrane-associated ring finger (C3HC4) 2, E3 ubiquitin protein ligase; membrane-associated RING finger protein 2; membrane-associated RING-CH protein II; RING finger protein 172; RING-type E3 ubiquitin transferase MARCH2; RING-type E3 ubiquitin transferase MARCHF2; RNF172
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0N8
  • Protein Name
    E3 ubiquitin-protein ligase MARCH2
  • Scientific Description
    MARCHF2 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MARCHF2 using anti-MARCHF2 antibody (A13497-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCHF2 antigen affinity purified polyclonal antibody (Catalog # A13497-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARCHF2 at approximately 27 kDa. The expected band size for MARCHF2 is at 27 kDa.
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