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Western blot analysis of LRRK2 expression in GFP-LRRK2 lysate.
Product group Antibodies
Boster Bio
Anti-LRRK2 Rabbit Monoclonal AntibodyM00221-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetLRRK2
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human thyroid, using LRRK2 Antibody(M00221-2) LRRK2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LRRK2 Antibody (M00221-2)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-LRRK2 Rabbit Monoclonal AntibodyM00221-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetLRRK2
  • SizePrice
Western blot analysis of LRRK2 in HEK293 cell lysate transfected with 3*Flag wild type, full length LRRK2 (M00221). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRK2 monoclonal antibody (Catalog # M00221) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRK2
Product group Antibodies
Boster Bio
Anti-LRRK2 Rabbit Monoclonal AntibodyM00221
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetLRRK2
  • SizePrice
Western blot analysis of Filamin A phosphorylation expression in WT-LRRK2 cell lysate treated LRRK2.
Product group Antibodies
Boster Bio
Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal AntibodyP00221
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetLRRK2
  • SizePrice
IHC analysis of Beclin 1 using anti-Beclin 1 antibody (PB9076).
Product group Antibodies
References
Boster Bio
Anti-Beclin 1/BECN1 Antibody Picoband(r)PB9076
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetBECN1
  • SizePrice
Anti-LRRK2 Picoband antibody, PB9281, Western blotting All lanes: Anti LRRK2 (PB9281) at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Mouse Brain Tissue Lysate at 50ug Lane 3: Rat Liver Tissue Lysate at 50ug Lane 4: U87 Whole Cell Lysate at 40ug Lane 5: NEURO Whole Cell Lysate at 40ug Lane 6: A549 Whole Cell Lysate at 40ug Lane 7: SMMC Whole Cell Lysate at 40ug Predicted bind size: 286KD Observed bind size: 286 KD
Product group Antibodies
Boster Bio
Anti-LRRK2 Antibody Picoband(r)PB9281
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetLRRK2
  • SizePrice
Anti-Beclin 1 antibody, PA1847, Western blotting Lane 1: HELA Cell Lysate Lane 2: SW620 Cell Lysate Lane 3: PANC Cell Lysate
Product group Antibodies
Boster Bio
Anti-Beclin 1/BECN1 Antibody Picoband(r)PA1847
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetBECN1
  • SizePrice
Western blot analysis of Beclin 1 expression in (1) HEK293 cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
Product group Antibodies
Boster Bio
Anti-Beclin 1 BECN1 Monoclonal AntibodyM00327
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBECN1
  • SizePrice
Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (PA1245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate, Lane 2: Human Placenta Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: MCF-7 Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: SW620 Whole Cell Lysate, Lane 7: MM231 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified polyclonal antibody (Catalog # PA1245-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 33KD. The expected band size for Cyclin D1 is at 33KD.
Product group Antibodies
Boster Bio
Anti-Cyclin D1/CCND1 Antibody Picoband(r)PA1245-2
ApplicationsFlow Cytometry, Western Blot
ReactivityChicken, Human, Rat
TargetCCND1
  • SizePrice
Anti-NR1H3 antibody, PA1246-1, Western blotting All lanes: Anti NR1H3 (PA1246-1) at 0.5ug/ml Lane 1: Rat Liver Tissue Lysate at 50ug Lane 2: Rat Lung Tissue Lysate at 50ug Lane 3: Rat Spleen Tissue Lysate at 50ug Lane 4: Rat Kidney Tissue Lysate at 50ug Lane 5: Human Placenta Tissue Lysate at 50ug Predicted bind size: 51KD Observed bind size: 51KD
Product group Antibodies
Boster Bio
Anti-LXR alpha/NR1H3 Antibody Picoband(r)PA1246-1
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetNR1H3
  • SizePrice
Anti-LXR alpha antibody, PA1246, Western blotting Lane 1: Rat Liver Tissue Lysate Lane 2: MCF-7 Cell Lysate Lane 3: HELA Cell Lysate
Product group Antibodies
Boster Bio
Anti-LXR alpha/NR1H3 Antibody Picoband(r)PA1246
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetNR1H3
  • SizePrice
Anti-TFPI2 antibody, PA1248, Western blotting Lane 1: MM453 Cell Lysate Lane 2: MM231 Cell Lysate Lane 3: HELA Cell Lysate Lane 4: HT1080 Cell Lysate Lane 5: JURKAT Cell Lysate
Product group Antibodies
Boster Bio
Anti-TFPI2 Antibody Picoband(r)PA1248
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTFPI2
  • SizePrice
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