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Immunohistochemical analysis of paraffin-embedded human kidney, using Apolipoprotein E Antibody.
Product group Antibodies
Boster Bio
Anti-Apolipoprotein E Monoclonal AntibodyM00015
IHC analysis of CDKN2A using anti-CDKN2A antibody (M00016) on human gastric cancer samples. CDKN2A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDKN2A Antibody (M00016) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-p16 INK Monoclonal AntibodyM00016
Immunofluorescent analysis of HeLa cells, using SIRT1 Antibody.
Product group Antibodies
Boster Bio
Anti-SIRT1 Monoclonal AntibodyM00018
Western blot analysis of CTLA4 (CD152) expression in NIH/3T3 cell lysate.
Product group Antibodies
Boster Bio
Anti-CTLA4 (CD152) Monoclonal AntibodyM00020
Western blot analysis of IL10 using anti-IL10 antibody (M00021) on Ramos cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # M00021) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IL10.
Product group Antibodies
Boster Bio
Anti-IL10 Monoclonal AntibodyM00021
Immunohistochemical analysis of paraffin-embedded human stomach cancer, using EGFR (ErbB 1) Antibody.
Product group Antibodies
Boster Bio
Anti-EGFR (ErbB 1) Monoclonal AntibodyM00023-2
Immunofluorescent analysis of HeLa cells, using AKT1 Antibody.
Product group Antibodies
Boster Bio
Anti-AKT1 Monoclonal AntibodyM00024-1
Immunofluorescent analysis of A549 cells, using MyD88 Antibody.
Product group Antibodies
Boster Bio
Anti-MyD88 Monoclonal AntibodyM00025
Immunofluorescent analysis of HeLa cells, using c-Myc Antibody.
Product group Antibodies
Boster Bio
Anti-c-Myc Monoclonal AntibodyM00026-1
Western blot analysis of JAK2 using anti-JAK2 antibody (M00027) in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-JAK2 Monoclonal AntibodyM00027
IHC analysis of MAPK1 using anti-MAPK1 antibody (M00030). MAPK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK1 Antibody (M00030) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-ERK2 Monoclonal AntibodyM00030
Immunofluorescent analysis of Jurkat cells, using CXCR4 Antibody.
Product group Antibodies
Boster Bio
Anti-CXCR4 Monoclonal AntibodyM00031
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