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Western blot analysis of Phospho-ATM (Ser1981) in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with Doxorubicin.
Product group Antibodies
Boster Bio
Anti-Phospho-ATM (S1981) Rabbit Monoclonal AntibodyP00014-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetATM
  • SizePrice
Western blot analysis of Phospho-SIRT1 (S47) expression in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP (P00018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 monoclonal antibody (Catalog # P00018-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1
Product group Antibodies
Boster Bio
Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal AntibodyP00018-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSIRT1
  • SizePrice
Western blot analysis of Phospho-SIRT1 (T530) expression in (1) 293T cell lysate; (2) 293T cell treated with Calyculin A.
Product group Antibodies
Boster Bio
Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal AntibodyP00018
ApplicationsWestern Blot
ReactivityHuman
TargetSIRT1
  • SizePrice
Western blot analysis of Phospho-EGFR (Y1086) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with EGF (P00023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
Product group Antibodies
Boster Bio
Anti-Phospho-EGFR (Y1086) Rabbit Monoclonal AntibodyP00023-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetEGFR
  • SizePrice
Western blot analysis of Phospho-EGFR (Y1173) expression in A431 cell lysate treated with EGF (P00023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
Product group Antibodies
Boster Bio
Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal AntibodyP00023-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetEGFR
  • SizePrice
Western blot analysis of Phospho-EGFR (Y1068) expression in (1) A431 cell lysate; (2) A431 cell treated with EGF lysate.
Product group Antibodies
References
Boster Bio
Anti-Phospho-EGFR (Y1092) Rabbit Monoclonal AntibodyP00023-3
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetEGFR
  • SizePrice
Western blot analysis of Phospho-EGFR (S695) expression in A431 cell lysate treated with EGF (P00023-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-4) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
Product group Antibodies
Boster Bio
Anti-Phospho-EGFR (S695) Rabbit Monoclonal AntibodyP00023-4
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetEGFR
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Phospho-EGFR (Y1092) Antibody (P00023) EGFR was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody (P00023)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Phospho-EGFR (Y1092) Rabbit Monoclonal AntibodyP00023
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Western blot analysis of Phospho-AKT1 (T450) expression in (1) 3T3 cell lysate treated with Lambda Phosphatase; (2) 3T3 cell lysate (P00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody (Catalog # P00024-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1
Product group Antibodies
Boster Bio
Anti-Phospho-AKT1 (T450) Rabbit Monoclonal AntibodyP00024-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Phospho-AKT1 (S129) expression in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-Phospho-AKT1 (S129) Rabbit Monoclonal AntibodyP00024-3
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate (P00024-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 monoclonal antibody (Catalog # P00024-4) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1
Product group Antibodies
References
Boster Bio
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal AntibodyP00024-4
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt(Ser473) Antibody.
Product group Antibodies
References
Boster Bio
Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal AntibodyP00024-5
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
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