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Western blot analysis of DKK1 using anti-DKK1 antibody (PA1462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (Catalog # PA1462) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1 at approximately 39KD. The expected band size for DKK1 is at 29KD.
Product group Antibodies
Boster Bio
Anti-Dickkopf-related protein 1 DKK1 Antibody Picoband(r)PA1462
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetDKK1
  • SizePrice
Anti-DLD antibody, PA1463, Western blotting Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Brain Tissue Lysate Lane 3: Rat Ovary Tissue Lysate Lane 4: Rat Testis Tissue Lysate Lane 5: SMMC Cell Lysate Lane 6: HELA Cell Lysate Lane 7: SMMC Cell Lysate Lane 8: JURKAT Cell Lysate
Product group Antibodies
Boster Bio
Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband(r)PA1463
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Rat
TargetDLD
  • SizePrice
Anti-FADD antibody, PA1464, Western blotting Lane 1: HELA Cell Lysate Lane 2: SMMC Cell Lysate Lane 3: SW620 Cell Lysate
Product group Antibodies
References
Boster Bio
Anti-FADD Antibody Picoband(r)PA1464
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetFADD
  • SizePrice
Anti-GDNF antibody, PA1465, Western blotting Lane 1: Recombinant Human GDNF Protein 10ng Lane 2: Recombinant Human GDNF Protein 5ng Lane 3: Recombinant Human GDNF Protein 2.5ng Lane 4: Recombinant Human GDNF Protein 1.25ng
Product group Antibodies
References
Boster Bio
Anti-GDNF Antibody Picoband(r)PA1465
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Rat
TargetGDNF
  • SizePrice
Anti-IL-2 antibody, PA1466, Western blotting Lane 1: Recombinant Mouse IL-2 Protein 10ng Lane 2: Recombinant Mouse IL-2 Protein 5ng Lane 3: Recombinant Mouse IL-2 Protein 2.5ng
Product group Antibodies
Boster Bio
Anti-Interleukin-2 IL2 Antibody Picoband(r)PA1466
ApplicationsWestern Blot
ReactivityMouse
TargetIl2
  • SizePrice
Anti-IL7 antibody, PA1467, IHC(P) IHC(P): Mouse Spleen Tissue
Product group Antibodies
Boster Bio
Anti-Interleukin-7 IL7 Antibody Picoband(r)PA1467
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse
TargetIl7
  • SizePrice
Anti-IL-12(p40) antibody, PA1468, Western blotting Lane 1: Recombinant Human IL-12 Protein 10ng Lane 2: Recombinant Human IL-12 Protein 5ng Lane 3: Recombinant Human IL-12 Protein 2.5ng
Product group Antibodies
Boster Bio
Anti-IL12 p40/IL12B Antibody Picoband(r)PA1468
ApplicationsWestern Blot
ReactivityEquine, Human, Rabbit
TargetIL12B
  • SizePrice
Anti-IL-13 antibody, PA1469, Western blotting All lanes: Anti IL-13 (PA1469) at 0.5ug/ml Lane 1: Recombinant Rat IL-13 Protein 5ng Lane 2: Recombinant Rat IL-13 Protein 2.5ng Predicted bind size: 13KD Observed bind size: 13KD
Product group Antibodies
Boster Bio
Anti-Interleukin-13 IL13 Antibody Picoband(r)PA1469
ApplicationsWestern Blot
ReactivityRat
TargetIl13
  • SizePrice
All lanes: Anti-IL23 P19 antibody, PA1470 Lane 1: Recombinant Mouse IL-23 Protein 10ng
Product group Antibodies
References
Boster Bio
Anti-IL23 P19/IL23A Antibody Picoband(r)PA1470
ApplicationsWestern Blot
ReactivityMouse
TargetIl23a
  • SizePrice
IHC analysis of PPID using anti-PPID antibody (PA1472).
Product group Antibodies
Boster Bio
Anti-PPID Antibody Picoband(r)PA1472
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetPPID
  • SizePrice
Anti-Resistin antibody, PA1473, Western blotting Lane 1: Recombinant Mouse Resistin Protein 10ng Lane 2: Recombinant Mouse Resistin Protein 5ng
Product group Antibodies
Boster Bio
Anti-Resistin/RETN Antibody Picoband(r)PA1473
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityMouse
TargetRetn
  • SizePrice
Anti-survivin antibody, PA1474, Western blotting Lane 1: HELA Cell Lysate Lane 2: JURKAT Cell Lysate Lane 3: CEM Cell Lysate Lane 4: COLO320 Cell Lysate
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474
ApplicationsWestern Blot
ReactivityCanine, Human
TargetBIRC5
  • SizePrice
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