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Are you looking for life science and diagnostic reagents? We offer one of the most extensive ranges in the Benelux. There are currently more than 5 million products in our webshop, which are manufactured by more than 130 suppliers. This includes both life science and diagnostic reagents. We hope to support your research with everything you need.

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Western blot analysis of Phospho-c-Myc (T58 + S62) in HEK293 cell lysate treated with EGF (P00026-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYC monoclonal antibody (Catalog # P00026-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYC
Product group Antibodies
Boster Bio
Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal AntibodyP00026-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rat
TargetMYC
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-c-Myc (S62) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-c-Myc (S62) Rabbit Monoclonal AntibodyP00026-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMYC
  • SizePrice
Western blot analysis of Phospho-c-Myc (T58) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin A and Okadaic Acid.
Product group Antibodies
Boster Bio
Anti-Phospho-c-Myc (T58) Rabbit Monoclonal AntibodyP00026
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetMYC
  • SizePrice
Western blot analysis of Phospho-STAT1 (Y701) expression in (1) A431 cell lysate; (2) A431 lysate cell treated with EGF.
Product group Antibodies
Boster Bio
Anti-Phospho-STAT1 (Y701) Rabbit Monoclonal AntibodyP00036
ApplicationsWestern Blot
ReactivityHuman
TargetSTAT1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded mouse spleen, using Phospho-Retinoblastoma (S807) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-Rb (S807) RB1 Rabbit Monoclonal AntibodyP00039-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRB1
  • SizePrice
Western blot analysis of Phospho-ER alpha (S118) expression in (1) MCF7 cell lysate; (2) MCF7 cell lysate treated with b-Estradiol and EGF.
Product group Antibodies
Boster Bio
Anti-Phospho-ER alpha (S118) ESR1 Rabbit Monoclonal AntibodyP00057
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetESR1
  • SizePrice
Immunofluorescent analysis of A549 cells treated with TGFβ, using Phospho-Smad3 (S423 + S425) Antibody.
Product group Antibodies
References
Boster Bio
Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal AntibodyP00059-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSMAD3
  • SizePrice
Western blot analysis of Phospho-B Raf (T401) expression in (1) PC-12 cell lysate; (2) PC-12 cell lysate treated with TPA.
Product group Antibodies
Boster Bio
Anti-Phospho-B Raf (T401) Rabbit Monoclonal AntibodyP00075
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetBRAF
  • SizePrice
Western blot analysis of Nrf2 phosphorylation expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-Nrf2 (S40) NFE2L2 Rabbit Monoclonal AntibodyP00078
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNFE2L2
  • SizePrice
Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate (P00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
Product group Antibodies
References
Boster Bio
Anti-Phospho-Smad2 (S250) Rabbit Monoclonal AntibodyP00090-1
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetSMAD2
  • SizePrice
Western blot analysis of Phospho-Smad2 (S255) expression in HeLa cell treated with Okadaic acid and Calyculin A lysate (P00090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
Product group Antibodies
References
Boster Bio
Anti-Phospho-Smad2 (S255) Rabbit Monoclonal AntibodyP00090
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD2
  • SizePrice
Western blot analysis of Phospho-PDGF Receptor beta (Y740) expression in NIH 3T3 treated with PDGF (P00096). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRB monoclonal antibody (Catalog # P00096) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFRB
Product group Antibodies
Boster Bio
Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal AntibodyP00096
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityMouse, Rat
TargetPDGFRB
  • SizePrice
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