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Figure 1. Western blotting validation for Anti-Phospho-Chk2 (S516) CHEK2 Antibody A00277S516 Western Blot (WB) analysis of specific cells using Phospho-Chk2 (S516) polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityHuman, Monkey
TargetCHEK2
  • SizePrice
Product group Antibodies
Boster Bio
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCHEK2
  • SizePrice
Figure 1. Western blotting validation for Anti-Phospho-Chk2 (T383) CHEK2 Antibody A00277T383 Western Blot (WB) analysis of HeLa cells using Phospho-Chk2 (T383) polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Monkey, Mouse, Rat
TargetCHEK2
  • SizePrice
Figure 1. Western blotting validation for Anti-Phospho-Chk2 (T387) CHEK2 Antibody A00277T387-1 Western Blot (WB) analysis of HeLa cells using Phospho-Chk2 (T387) polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCHEK2
  • SizePrice
Product group Antibodies
Boster Bio
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCHEK2
  • SizePrice
Figure 1. Western blotting validation for Anti-Chk2 (phospho-T432) CHEK2 Antibody A00277T432 Western blot (WB) analysis of p-Chk2 (T432) polyclonal antibody at 1:500 dilution Lane1:Jurkat whole cell lysate Lane2:H9C2 whole cell lysate Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetCHEK2
  • SizePrice
Figure 2. Immunohistochemistry validation of CHEK2 using Anti-Chk2 (phospho-T68) CHEK2 Antibody (A00277T68). Immunohistochemistry (IHC) analyzes of p-Chk2 (T68) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. For more protocol information of IHC
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCHEK2
  • SizePrice
Figure 1. Immunocytochemistry staining of CXCL10 using Anti-IP10 CXCL10 Antibody (A00278). ICC staining IP10 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCXCL10
  • SizePrice
Figure 1. Western blot analysis of NF2/Merlin using anti-NF2/Merlin antibody (A00279-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2/Merlin antigen affinity purified polyclonal antibody (Catalog # A00279-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF2/Merlin at approximately 69 kDa. The expected band size for NF2/Merlin is at 70 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNF2
  • SizePrice
Figure 1. Western blot analysis of NF2/Merlin using anti-NF2/Merlin antibody (A00279-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2/Merlin antigen affinity purified polyclonal antibody (Catalog # A00279-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF2/Merlin at approximately 69 kDa. The expected band size for NF2/Merlin is at 70 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNF2
  • SizePrice
Figure 1. Western blot analysis of NF2/Merlin using anti-NF2/Merlin antibody (A00279-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2/Merlin antigen affinity purified polyclonal antibody (Catalog # A00279-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF2/Merlin at approximately 69 kDa. The expected band size for NF2/Merlin is at 70 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNF2
  • SizePrice
Figure 1. Western blot analysis of NF2/Merlin using anti-NF2/Merlin antibody (A00279-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2/Merlin antigen affinity purified polyclonal antibody (Catalog # A00279-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF2/Merlin at approximately 69 kDa. The expected band size for NF2/Merlin is at 70 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNF2
  • SizePrice

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