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Western blot analysis of Ambra1 in 3T3 cell lysate with Ambra1 antibody at 1 microg/mL.
Product group Antibodies
Boster Bio
Anti-Ambra1 AntibodyA03799
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityEquine, Human, Mouse, Porcine, Rat
TargetAMBRA1
  • SizePrice
Figure 1. Western blotting validation for Anti-EIF2B5 Antibody A03801 Western blot analysis of extracts of various cell lines
Product group Antibodies
Boster Bio
Anti-EIF2B5 AntibodyA03801
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetEIF2B5
  • SizePrice
Western blot analysis of Aldh5A1 in human liver tissue lysate with Aldh5A1 antibody at (A) 0.25 and (B) 0.5 microg/mL.
Product group Antibodies
Boster Bio
Anti-Aldh5A1 AntibodyA03802
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetALDH5A1
  • SizePrice
Figure 1. Western blotting validation for Anti-Sulfotransferase 1A3 SULT1A3 Antibody A03804 Western blot analysis of extracts of various cell lines
Product group Antibodies
Boster Bio
Anti-Sulfotransferase 1A3 SULT1A3 AntibodyA03804
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSULT1A3
  • SizePrice
Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Product group Antibodies
Boster Bio
Anti-HIF3/HIF3A Antibody Picoband(r)A03805-3-BIOTIN
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHIF3A
  • SizePrice
Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Product group Antibodies
Boster Bio
Anti-HIF3/HIF3A Antibody Picoband(r)A03805-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHIF3A
  • SizePrice
Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Product group Antibodies
Boster Bio
Anti-HIF3/HIF3A Antibody Picoband(r)A03805-3-CY3
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHIF3A
  • SizePrice
Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Product group Antibodies
Boster Bio
Anti-HIF3/HIF3A Antibody Picoband(r)A03805-3-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHIF3A
  • SizePrice
Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Product group Antibodies
Boster Bio
Anti-HIF3/HIF3A Antibody Picoband(r)A03805-3-DYLIGHT550
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHIF3A
  • SizePrice
Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Product group Antibodies
Boster Bio
Anti-HIF3/HIF3A Antibody Picoband(r)A03805-3-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHIF3A
  • SizePrice
Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Product group Antibodies
Boster Bio
Anti-HIF3/HIF3A Antibody Picoband(r)A03805-3-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHIF3A
  • SizePrice
Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
Product group Antibodies
Boster Bio
Anti-HIF3/HIF3A Antibody Picoband(r)A03805-3-HRP
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetHIF3A
  • SizePrice
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