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Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using MHC class 1 Antibody.
Product group Antibodies
Boster Bio
Anti-MHC class 1 Rabbit Monoclonal AntibodyM00194-1
Western blot analysis of MHC class I expression in Raji cell lysate.
Product group Antibodies
Boster Bio
Anti-MHC class I Rabbit Monoclonal AntibodyM00194-2
Western blot analysis of ITCH expression in K562 cell lysate using anti-ITCH antibody (M00195). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITCH antigen affinity purified polyclonal antibody (Catalog # M00195) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-ITCH Monoclonal AntibodyM00195
Western blot analysis of CD80 expression in Raji cell lysate.
Product group Antibodies
Boster Bio
Anti-CD80/B7 1 Rabbit Monoclonal AntibodyM00196
Western blot analysis of TLR9 expression in Raji cell lysate (M00198). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR9 monoclonal antibody (Catalog # M00198) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR9
Product group Antibodies
Boster Bio
Anti-TLR9 Rabbit Monoclonal AntibodyM00198
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
Product group Antibodies
Boster Bio
Anti-Wilms Tumor Protein Rabbit Monoclonal AntibodyM00199-1
Western blot analysis of FGFR3 expression in HeLa cell lysate (M00200-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR3 monoclonal antibody (Catalog # M00200-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR3
Product group Antibodies
Boster Bio
Anti-FGFR3 Rabbit Monoclonal AntibodyM00200-1
Western blot analysis of FGFR3 expression in (1) A549 cell lysate; (2) Mouse brain lysate.
Product group Antibodies
Boster Bio
Anti-FGFR3 Rabbit Monoclonal AntibodyM00200
Western blot analysis of RAN expression in (1)HeLa cell lysate; (2)RAW264.7 cell lysate using anti-RAN antibody (M00204). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAN antigen affinity purified polyclonal antibody (Catalog # M00204) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RAN.
Product group Antibodies
Boster Bio
Anti-Ran Monoclonal AntibodyM00204
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody (M00207-1) RHOA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RHOA Antibody (M00207-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Rho A + B + C Rabbit Monoclonal AntibodyM00207-1
Western blot analysis of Rho expression in HeLa cell lysate (M00207-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHOA monoclonal antibody (Catalog # M00207-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHOA
Product group Antibodies
Boster Bio
Anti-Rho Rabbit Monoclonal AntibodyM00207-2
Western blot analysis of Rho A expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (M00207). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHOA monoclonal antibody (Catalog # M00207) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHOA
Product group Antibodies
Boster Bio
Anti-Rho A Rabbit Monoclonal AntibodyM00207
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