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Western blot analysis of CDK1 in HeLa cell lysate (M00209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
Product group Antibodies
Boster Bio
Anti-CDK1/Cdc2 Rabbit Monoclonal AntibodyM00209-1
Immunohistochemical analysis of paraffin-embedded human colon, using CDK1 Antibody(M00209-2) CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (M00209-2)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CDK1/Cdc2 Rabbit Monoclonal AntibodyM00209-2
Western blot analysis of CDK1 expression in HeLa cell lysate (M00209-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-3) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
Product group Antibodies
Boster Bio
Anti-CDK1/Cdc2 Rabbit Monoclonal AntibodyM00209-3
Western blot analysis of CDK1 expression in (1)A431 cell lysate;(2)HeLa cell lysate (M00209-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-4) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
Product group Antibodies
Boster Bio
Anti-CDK1/Cdc2 Rabbit Monoclonal AntibodyM00209-4
Western blot analysis of Vitamin D expression in (1) HeLa cell lysate; (2) Mouse kidney lysate (M00210). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDR monoclonal antibody (Catalog # M00210) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDR
Product group Antibodies
Boster Bio
Anti-Vitamin D Receptor Rabbit Monoclonal AntibodyM00210
Western blot analysis of Myelin Basic Protein expression in Human fetal brain lysate.
Product group Antibodies
Boster Bio
Anti-Myelin Basic Protein Rabbit Monoclonal AntibodyM00211
Western blot analysis of GFAP expression in Rat brain lysate.
Product group Antibodies
Boster Bio
Anti-GFAP Rabbit Monoclonal AntibodyM00213-1
Immunohistochemical analysis of paraffin-embedded human glioma, using GFAP Antibody.
Product group Antibodies
Boster Bio
Anti-GFAP Rabbit Monoclonal AntibodyM00213-2
IHC analysis of IL2RA using anti-IL2RA antibody (M00214-1) on paraffin-embedded human tonsil. IL2RA was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL2RA Antibody (M00214-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CD25 Monoclonal AntibodyM00214-1
Immunohistochemical analysis of paraffin-embedded mouse testis, using Alpha Synuclein Antibody(M00215-1) SNCA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SNCA Antibody (M00215-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Alpha Synuclein Rabbit Monoclonal AntibodyM00215-1
Western blot analysis of alpha + beta Synuclein expression in Mouse brain tissue lysate.
Product group Antibodies
Boster Bio
Anti-alpha + beta Synuclein Rabbit Monoclonal AntibodyM00215
Western blot analysis of FTO expression in (1) 293T cell lysate; (2) HeLa cell lysate; (3) SiHa cell lysate; (4) Daudi cell lysate; (5) HepG2 cell lysate; (6) T47D cell lysate.
Product group Antibodies
Boster Bio
Anti-FTO Monoclonal AntibodyM00219
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