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Western blot analysis of HLA-DQA1 expression in human spleen lysate.
Product group Antibodies
Boster Bio
Anti-HLA-DQA1 Monoclonal AntibodyM00232-1
Western blot analysis of Hemoglobin subunit alpha expression in K562 cell lysate.
Product group Antibodies
Boster Bio
Anti-Hemoglobin subunit alpha Rabbit Monoclonal AntibodyM00233
Western blot analysis of XBP1 expression in Jurkat cell lysate (M00234). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XBP1 monoclonal antibody (Catalog # M00234) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XBP1
Product group Antibodies
Boster Bio
Anti-XBP1 Rabbit Monoclonal AntibodyM00234
Western blot analysis of Vimentin expression in HEK293 cell lysate.
Product group Antibodies
Boster Bio
Anti-Vimentin Rabbit Monoclonal AntibodyM00235-1
Western blot analysis of PGC1 alpha/beta expression in (1) HeLa cell lysate; (2) NIH 3T3 cell lysate; (3) C6 cell lysate.
Product group Antibodies
Boster Bio
Anti-PGC1 alpha Rabbit Monoclonal AntibodyM00236
Western blot analysis of Fadd expression in Raw264.7 cell lysate using anti-Fadd antibody (M00237-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fadd antigen affinity purified polyclonal antibody (Catalog # M00237-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Fadd.
Product group Antibodies
Boster Bio
Anti-FADD Monoclonal AntibodyM00237-1
Western blot analysis of FADD expression in A431 cell lysate using anti-FADD antibody (M00237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # M00237) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for FADD.
Product group Antibodies
Boster Bio
Anti-FADD Monoclonal AntibodyM00237
Western blot analysis of CD80 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-SOD1/Cu Zn Sod Rabbit Monoclonal AntibodyM00238
Western blot analysis of GPR49 expression in Human fetal skeletal muscle lysate.
Product group Antibodies
Boster Bio
Anti-LGR5/GPR49 Rabbit Monoclonal AntibodyM00239
Western blot analysis of ATG5 expression in Raji cell lysate (M00240). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG5 monoclonal antibody (Catalog # M00240) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG5
Product group Antibodies
Boster Bio
Anti-ATG5/Apg5 Rabbit Monoclonal AntibodyM00240
Immunohistochemical analysis of paraffin-embedded rat lung, using Histone H2AX Antibody(M00241-1) H2AFX was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-H2AFX Antibody (M00241-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Histone H2A.X Rabbit Monoclonal AntibodyM00241-1
Western blot analysis of DDX58 expression in Jurkat cell lysate using anti-DDX58 antibody (M00244). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX58 antigen affinity purified polyclonal antibody (Catalog # M00244) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for DDX58.
Product group Antibodies
Boster Bio
Anti-DDX58 Monoclonal AntibodyM00244
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