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Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A549 whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human Raji whole cell lysates; Lane 5:huamn Caco-2 whole cell lysates; Lane 6: huamn Jurkat whole cell lysates; Lane 7: mouse brain tissue lysates; Lane 8: rat brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
Product group Antibodies
Boster Bio
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband(r) (monoclonal, 6G5)M01141-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAZ
  • SizePrice
Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A549 whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human Raji whole cell lysates; Lane 5:huamn Caco-2 whole cell lysates; Lane 6: huamn Jurkat whole cell lysates; Lane 7: mouse brain tissue lysates; Lane 8: rat brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
Product group Antibodies
Boster Bio
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband(r) (monoclonal, 6G5)M01141-DYLIGHT550
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAZ
  • SizePrice
Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A549 whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human Raji whole cell lysates; Lane 5:huamn Caco-2 whole cell lysates; Lane 6: huamn Jurkat whole cell lysates; Lane 7: mouse brain tissue lysates; Lane 8: rat brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
Product group Antibodies
Boster Bio
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband(r) (monoclonal, 6G5)M01141-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAZ
  • SizePrice
Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A549 whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human Raji whole cell lysates; Lane 5:huamn Caco-2 whole cell lysates; Lane 6: huamn Jurkat whole cell lysates; Lane 7: mouse brain tissue lysates; Lane 8: rat brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
Product group Antibodies
Boster Bio
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband(r) (monoclonal, 6G5)M01141-FITC
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAZ
  • SizePrice
Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A549 whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human Raji whole cell lysates; Lane 5:huamn Caco-2 whole cell lysates; Lane 6: huamn Jurkat whole cell lysates; Lane 7: mouse brain tissue lysates; Lane 8: rat brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
Product group Antibodies
Boster Bio
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband(r) (monoclonal, 6G5)M01141-HRP
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAZ
  • SizePrice
Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A549 whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human Raji whole cell lysates; Lane 5:huamn Caco-2 whole cell lysates; Lane 6: huamn Jurkat whole cell lysates; Lane 7: mouse brain tissue lysates; Lane 8: rat brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
Product group Antibodies
Boster Bio
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband(r) (monoclonal, 6G5)M01141-IFLUOR647
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAZ
  • SizePrice
Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A549 whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human Raji whole cell lysates; Lane 5:huamn Caco-2 whole cell lysates; Lane 6: huamn Jurkat whole cell lysates; Lane 7: mouse brain tissue lysates; Lane 8: rat brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
Product group Antibodies
Boster Bio
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband(r) (monoclonal, 6G5)M01141-PE
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAZ
  • SizePrice
Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A549 whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human Raji whole cell lysates; Lane 5:huamn Caco-2 whole cell lysates; Lane 6: huamn Jurkat whole cell lysates; Lane 7: mouse brain tissue lysates; Lane 8: rat brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
Product group Antibodies
Boster Bio
Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband(r) (monoclonal, 6G5)M01141
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetYWHAZ
  • SizePrice
Figure 1. Western blot analysis of HP1 Gamma using anti-HP1 Gamma antibody (M01142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U-937 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 Gamma antigen affinity purified monoclonal antibody (Catalog # M01142-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 Gamma at approximately 21 kDa. The expected band size for HP1 Gamma is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-HP1 gamma Rabbit Monoclonal AntibodyM01142-1-30UL
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCBX3
  • SizePrice
Figure 1. Western blot analysis of HP1 Gamma using anti-HP1 Gamma antibody (M01142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U-937 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 Gamma antigen affinity purified monoclonal antibody (Catalog # M01142-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 Gamma at approximately 21 kDa. The expected band size for HP1 Gamma is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-HP1 gamma Rabbit Monoclonal AntibodyM01142-1
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCBX3
  • SizePrice
Product group Antibodies
Boster Bio
Anti-CBX3 (5G10) monoclonal AntibodyM01142
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetCBX3
  • SizePrice
Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).
Product group Antibodies
Boster Bio
Anti-IL1 alpha (IL1A) Mouse Monoclonal Antibody [Clone ID: OTI2F8]M01144-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetIL1A
  • SizePrice
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