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Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-3). VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-VEGF Receptor 3 Antibody (A01276-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-VEGF Receptor 3/FLT4 Antibody Picoband(r)A01276-3-DYLIGHT594
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetFLT4
  • SizePrice
Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-3). VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-VEGF Receptor 3 Antibody (A01276-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-VEGF Receptor 3/FLT4 Antibody Picoband(r)A01276-3-FITC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetFLT4
  • SizePrice
Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-3). VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-VEGF Receptor 3 Antibody (A01276-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-VEGF Receptor 3/FLT4 Antibody Picoband(r)A01276-3-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetFLT4
  • SizePrice
Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-3). VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-VEGF Receptor 3 Antibody (A01276-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-VEGF Receptor 3/FLT4 Antibody Picoband(r)A01276-3-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetFLT4
  • SizePrice
Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-3). VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-VEGF Receptor 3 Antibody (A01276-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-VEGF Receptor 3/FLT4 Antibody Picoband(r)A01276-3-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetFLT4
  • SizePrice
Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-3). VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-VEGF Receptor 3 Antibody (A01276-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-VEGF Receptor 3/FLT4 Antibody Picoband(r)A01276-3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetFLT4
  • SizePrice
Figure 2. Immunohistochemistry validation of ARF1 using Anti-ADP-ribosylation factor 1 ARF1 Antibody (A01279-1). Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin. For more protocol information of IHC
Product group Antibodies
Boster Bio
Anti-ADP-ribosylation factor 1 ARF1 AntibodyA01279-1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetARF1
  • SizePrice
Figure 1. Western blotting validation for Anti-ADP-ribosylation factor 1 ARF1 Antibody A01279 Western blot analysis of extracts of various cells
Product group Antibodies
Boster Bio
Anti-ADP-ribosylation factor 1 ARF1 AntibodyA01279
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetARF1
  • SizePrice
Product group Antibodies
Boster Bio
Anti-HSP60 (T547) HSPD1 AntibodyA01280T547
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPD1
  • SizePrice
Western Blot (WB) analysis of 4T1 cells using CD81 Polyclonal antibody.
Product group Antibodies
References
Boster Bio
Anti-CD81 antigen CD81 AntibodyA01281-1
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCD81
  • SizePrice
Figure 1. Western blot analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse raw264.7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1 antigen affinity purified polyclonal antibody (Catalog # A01281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1 at approximately 22KD. The expected band size for TAPA1 is at 22KD.
Product group Antibodies
Boster Bio
Anti-TAPA1/CD81 Antibody Picoband(r)A01281-2-10UG
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetCD81
  • SizePrice
Figure 1. Western blot analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse raw264.7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1 antigen affinity purified polyclonal antibody (Catalog # A01281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1 at approximately 22KD. The expected band size for TAPA1 is at 22KD.
Product group Antibodies
Boster Bio
Anti-TAPA1/CD81 Antibody Picoband(r)A01281-2-APC
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetCD81
  • SizePrice
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