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Western blot analysis of BTK expression in Daudi cell lysate (M00245). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTK monoclonal antibody (Catalog # M00245) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BTK
Product group Antibodies
Boster Bio
Anti-BTK Rabbit Monoclonal AntibodyM00245
Western blot analysis of Aurora A expression in HepG2 cell lysate (M00246). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AURKA monoclonal antibody (Catalog # M00246) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AURKA
Product group Antibodies
Boster Bio
Anti-Aurora A Rabbit Monoclonal AntibodyM00246
Western blot analysis of CD147 expression in Mouse kidney lysate (M00248-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BSG monoclonal antibody (Catalog # M00248-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BSG
Product group Antibodies
Boster Bio
Anti-CD147 Rabbit Monoclonal AntibodyM00248-1
Immunohistochemical analysis of paraffin-embedded human liver, using C Reactive Protein Antibody.
Product group Antibodies
Boster Bio
Anti-C Reactive Protein Rabbit Monoclonal AntibodyM00249
Western blot analysis of ACE1 expression in human fetal kidney lysate.
Product group Antibodies
Boster Bio
Anti-ACE1 Monoclonal AntibodyM00251
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma, using FoxO3a Antibody.
Product group Antibodies
Boster Bio
Anti-FoxO3a Rabbit Monoclonal AntibodyM00252-1
Western blot analysis of FOXO3A expression in MCF-7 cell lysate.
Product group Antibodies
Boster Bio
Anti-FoxO3a Rabbit Monoclonal AntibodyM00252
Immunohistochemical analysis of paraffin-embedded human tonsil, using Ki67 Antibody (M00254) MKI67 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MKI67 Antibody (M00254)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Ki67 Rabbit Monoclonal AntibodyM00254
Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1 Antibody.
Product group Antibodies
Boster Bio
Anti-HDAC1 Rabbit Monoclonal AntibodyM00256
Western blot analysis of E2F1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
Product group Antibodies
Boster Bio
Anti-E2F1 Rabbit Monoclonal AntibodyM00257
Immunohistochemical analysis of paraffin-embedded human testis, using NAK/TBK1 (N-term) Antibody(M00261) TBK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TBK1 Antibody (M00261)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-NAK/TBK1 (N-term) Rabbit Monoclonal AntibodyM00261
Western blot analysis of IFIH1 expression in THP1 cell lysate using anti-IFIH1 antibody (M00263-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFIH1 antigen affinity purified polyclonal antibody (Catalog # M00263-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IFIH1.
Product group Antibodies
Boster Bio
Anti-MDA5 Antibody (Monoclonal, 1B6)M00263-1
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