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Western blot analysis of LARS using anti-LARS antibody (A01109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARS antigen affinity purified polyclonal antibody (Catalog # A01109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARS at approximately 134KD. The expected band size for LARS is at 134KD.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Leucyl tRNA synthetase/LARS AntibodyA01109
Western blot analysis of Steroid sulfatase using anti-Steroid sulfatase antibody (A01198-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Steroid sulfatase antigen affinity purified polyclonal antibody (Catalog # A01198-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Steroid sulfatase at approximately 65KD. The expected band size for Steroid sulfatase is at 65KD.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Steroid sulfatase/STS AntibodyA01198-1
Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody (A01313). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody (Catalog # A01313) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 65KD. The expected band size for Heparanase 1 is at 61KD.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Heparanase 1 AntibodyA01313
Protein IDQ6YGZ1
Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (A01376-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-DNA Polymerase iota/POLI AntibodyA01376-1
Western blot analysis of HK2 using anti-HK2 antibody (A01389).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Hexokinase II Picoband AntibodyA01389
Protein IDP52789
Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (A01626-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Tryptophan Hydroxylase/TPH1 AntibodyA01626-1
IHC analysis of CKB using anti-CKB antibody (A01695-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Creatine kinase B type/CKB AntibodyA01695-1
IHC analysis of MYLK using anti-MYLK antibody (A01697-2).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Myosin light chain kinase/MYLK AntibodyA01697-2
Western blot analysis of CES1 using anti-CES1 antibody (A01741-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Liver Carboxylesterase 1/CES1 AntibodyA01741-1
IHC analysis of SOD3 using anti-SOD3 antibody (A01784-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Superoxide Dismutase 3/SOD3 AntibodyA01784-1
IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (A01884). Xanthine Oxidase was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Xanthine Oxidase Antibody (A01884) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Xanthine Oxidase AntibodyA01884
Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-12 Lipoxygenase Picoband AntibodyA02275-1
Protein IDP18054
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