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Western blot analysis of FOXA1 expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-FOXA1/Hnf 3 Alpha Rabbit Monoclonal AntibodyM00266-1
Western blot analysis of IRS1 expression in HEK293 cell lysate (M00268). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRS1 monoclonal antibody (Catalog # M00268) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRS1
Product group Antibodies
Boster Bio
Anti-IRS1 Rabbit Monoclonal AntibodyM00268
Western blot analysis of MiTF expression in A375 cell lysate (M00269-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MITF monoclonal antibody (Catalog # M00269-2) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MITF
Product group Antibodies
Boster Bio
Anti-MiTF Rabbit Monoclonal AntibodyM00269-2
Western blot analysis of VWF expression in human serum lysate (M00270-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VWF monoclonal antibody (Catalog # M00270-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VWF
Product group Antibodies
Boster Bio
Anti-VWF/Von Willebrand Factor Rabbit Monoclonal AntibodyM00270-1
Western blot analysis of PAX6 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-PAX6 Rabbit Monoclonal AntibodyM00273-2
Western blot analysis of PAX6 expression in Human brain lysate.
Product group Antibodies
Boster Bio
Anti-PAX6 Rabbit Monoclonal AntibodyM00273-3
Western blot analysis of Chk2 expression in (1) HeLa cell lysate; (2) 293T cell lysate (M00277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK2 monoclonal antibody (Catalog # M00277) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK2
Product group Antibodies
Boster Bio
Anti-Chk2 Rabbit Monoclonal AntibodyM00277
Western blot analysis of IP10 expression in IP10 recombinant protein lysate.
Product group Antibodies
Boster Bio
Anti-IP10 Rabbit Monoclonal AntibodyM00278
Western blot analysis of NF2 expression in (1) HeLa cell lysate; (2) PC3 cell lysate using anti-NF2 antibody (M00279). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2 antigen affinity purified polyclonal antibody (Catalog # M00279) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NF2.
Product group Antibodies
Boster Bio
Anti-NF2 / Merlin Monoclonal AntibodyM00279
Western blot analysis of CSF3R expression in Caco-2 cell lysate using anti-CSF3R antibody (M00281). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF3R antigen affinity purified polyclonal antibody (Catalog # M00281) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CSF3R.
Product group Antibodies
Boster Bio
Anti-GCSF Receptor (CD114) Monoclonal AntibodyM00281
Immunohistochemical analysis of paraffin-embedded human kidney, using NF-κB (p105/p50) Antibody.
Product group Antibodies
Boster Bio
Anti-NF-Kappa B (p105/p50) Rabbit Monoclonal AntibodyM00283
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-κB p65 Antibody.
Product group Antibodies
Boster Bio
Anti-NF-Kappa B p65 Rabbit Monoclonal AntibodyM00284
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