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Figure 1. Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGADD45G
  • SizePrice
Figure 1. Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGADD45G
  • SizePrice
Figure 1. Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGADD45G
  • SizePrice
Figure 1. Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGADD45G
  • SizePrice
Figure 1. Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGADD45G
  • SizePrice
Figure 1. Western blotting validation for Anti-GADD 4 gamma GADD45G Antibody A04681 Western Blot (WB) analysis of specific cells using GADD 45gamma polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGADD45G
  • SizePrice
Figure 1. Immunohistochemistry validation of GADD45G using Anti-GADD 45 Gamma (S123) GADD45G Antibody (A04681S123). Immunohistochemistry (IHC) analyzes of GADD 45gamma (S123) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. For more protocol information of IHC
Product group Antibodies
Boster Bio
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGADD45G
  • SizePrice
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCOQ7
  • SizePrice
Western blot analysis of Dapper homolog 1. Anti-Dapper homolog 1 antibody was used at 4 &mu
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetDACT1
  • SizePrice
Figure 2. Immunohistochemistry validation of ORM2 using Anti-AGP-2 ORM2 Antibody (A04684). Immunohistochemistry (IHC) analyzes of AGP-2 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetORM2
  • SizePrice
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSPINT1
  • SizePrice
Figure 1. Western blotting validation for Anti-REEP1 Antibody A04687 Western blot analysis of extracts of various tissues
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetREEP1
  • SizePrice

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