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Western blot analysis of MMP2 expression in (1)L6 cell lysate;(2)HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-MMP2 Rabbit Monoclonal AntibodyM00286-1
Western blot analysis of MMP2 expression in L6 cell lysate.
Product group Antibodies
Boster Bio
Anti-MMP2 Monoclonal AntibodyM00286-3
Western blot analysis of G6PD expression in MCF7 cell lysate.
Product group Antibodies
Boster Bio
Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal AntibodyM00287
Western blot analysis of TTR expression in human serum lysate (M00290-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTR monoclonal antibody (Catalog # M00290-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTR
Product group Antibodies
Boster Bio
Anti-TTR/Prealbumin Rabbit Monoclonal AntibodyM00290-1
Western blot analysis of Prealbumin gamma expression in human fetal kidney lysate (M00290). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTR monoclonal antibody (Catalog # M00290) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTR
Product group Antibodies
Boster Bio
Anti-Prealbumin Rabbit Monoclonal AntibodyM00290
Immunohistochemical analysis of paraffin-embedded human kidney, using MEK1/2 Antibody(M00292-1) MAP2K1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K1 Antibody (M00292-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-MEK1/2 Rabbit Monoclonal AntibodyM00292-1
Western blot analysis of MEK1 expression in (1) A431 cell lysate;(2) HeLa cell lysate (M00292-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
Product group Antibodies
Boster Bio
Anti-MEK1 Rabbit Monoclonal AntibodyM00292-2
Western blot analysis of MEK1 expression in A431 cell lysate (M00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
Product group Antibodies
Boster Bio
Anti-MEK1 Rabbit Monoclonal AntibodyM00292
Western blot analysis of Ret expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
Anti-Ret Rabbit Monoclonal AntibodyM00293
Western blot analysis of CXCR3 expression in K562 cell lysate using anti-CXCR3 antibody (M00295). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR3 antigen affinity purified polyclonal antibody (Catalog # M00295) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CXCR3.
Product group Antibodies
Boster Bio
Anti-CXCR3 Monoclonal AntibodyM00295
Western blot analysis of Mitofusin 2 expression in (1) HeLa cell lysate; (2) Mouse kidney lysate.
Product group Antibodies
Boster Bio
Anti-Mitofusin 2 Rabbit Monoclonal AntibodyM00461
Western blot analysis of TLR5 expression in HeLa cell lysate (M00462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR5 monoclonal antibody (Catalog # M00462) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR5
Product group Antibodies
Boster Bio
Anti-TLR5 Rabbit Monoclonal AntibodyM00462
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