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Western blot analysis of VASP expression in HepG2 cell lysate (M00303). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VASP monoclonal antibody (Catalog # M00303) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VASP
Product group Antibodies
Boster Bio
Anti-VASP Rabbit Monoclonal AntibodyM00303
Western blot analysis of POMC using anti-POMC antibody (M00305-1) in mouse pituitary lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMC antigen affinity purified polyclonal antibody (Catalog # M00305-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-POMC Monoclonal AntibodyM00305-1
Western blot analysis of IFNAR1 expression in K562 cell lysate.
Product group Antibodies
Boster Bio
Anti-IFNAR1/Beta R1 Rabbit Monoclonal AntibodyM00306-1
Western blot analysis of Interferon Receptor alpha expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
Anti-Interferon Receptor alpha Rabbit Monoclonal AntibodyM00306
Western blot analysis of CETP expression in human plasma lysate.
Product group Antibodies
Boster Bio
Anti-CETP Rabbit Monoclonal AntibodyM00309
Western blot analysis of DDIT3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
Product group Antibodies
Boster Bio
Anti-DDIT3/Chop Rabbit Monoclonal AntibodyM00311
Western blot analysis of STIM1 using anti-STIM1 antibody (PB9406, Left) and anti-STIM1 antibody (M00312, Right).
Product group Antibodies
Boster Bio
Anti-Stromal interaction molecule 1 Rabbit Monoclonal AntibodyM00312
Western blot analysis of Bmi1 expression in (1) K562 cell lysate; (2) PC-12 cell lysate (M00313). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMI1 monoclonal antibody (Catalog # M00313) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMI1
Product group Antibodies
Boster Bio
Anti-Bmi1 Rabbit Monoclonal AntibodyM00313
Western blot analysis of Dnmt3b expression in (1) A549 cell lysate; (2) A431 cell lysate (M00319). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B monoclonal antibody (Catalog # M00319) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal AntibodyM00319
Western blot analysis of TPO using anti-TPO antibody in Human thyroid lysate. (M00320). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPO antigen affinity purified polyclonal antibody (Catalog # M00320) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-TPO Monoclonal AntibodyM00320
Immunohistochemical analysis of paraffin-embedded human breast cancer, using BMP4 Antibody(M00321) BMP4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BMP4 Antibody (M00321)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-BMP4 Rabbit Monoclonal AntibodyM00321
Western blot analysis of BACE1 expression in SH-SY5Y cell lysate (M00322). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BACE1 monoclonal antibody (Catalog # M00322) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BACE1
Product group Antibodies
Boster Bio
Anti-BACE1/Bace Rabbit Monoclonal AntibodyM00322
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