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Figure 1. Western blot analysis of BRCC36/BRCC3 using anti-BRCC36/BRCC3 antibody (A04690-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCC36/BRCC3 antigen affinity purified polyclonal antibody (Catalog # A04690-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCC36/BRCC3 at approximately 36 kDa. The expected band size for BRCC36/BRCC3 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-BRCC36/BRCC3 Antibody Picoband(r)A04690-1-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetBRCC3
  • SizePrice
Figure 1. Western blot analysis of BRCC36/BRCC3 using anti-BRCC36/BRCC3 antibody (A04690-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCC36/BRCC3 antigen affinity purified polyclonal antibody (Catalog # A04690-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCC36/BRCC3 at approximately 36 kDa. The expected band size for BRCC36/BRCC3 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-BRCC36/BRCC3 Antibody Picoband(r)A04690-1-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetBRCC3
  • SizePrice
Figure 1. Western blot analysis of BRCC36/BRCC3 using anti-BRCC36/BRCC3 antibody (A04690-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCC36/BRCC3 antigen affinity purified polyclonal antibody (Catalog # A04690-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCC36/BRCC3 at approximately 36 kDa. The expected band size for BRCC36/BRCC3 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-BRCC36/BRCC3 Antibody Picoband(r)A04690-1-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetBRCC3
  • SizePrice
Figure 1. Western blot analysis of BRCC36/BRCC3 using anti-BRCC36/BRCC3 antibody (A04690-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCC36/BRCC3 antigen affinity purified polyclonal antibody (Catalog # A04690-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCC36/BRCC3 at approximately 36 kDa. The expected band size for BRCC36/BRCC3 is at 36 kDa.
Product group Antibodies
Boster Bio
Anti-BRCC36/BRCC3 Antibody Picoband(r)A04690-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetBRCC3
  • SizePrice
Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 microg/mL.
Product group Antibodies
Boster Bio
Anti-BRCC36 AntibodyA04690
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Rat
TargetBRCC3
  • SizePrice
Figure 1. Western blot analysis of HSF2 using anti-HSF2 antibody (A04692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # A04692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-HSF2 Antibody Picoband(r)A04692-10UG
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHSF2
  • SizePrice
Figure 1. Western blot analysis of HSF2 using anti-HSF2 antibody (A04692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # A04692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-HSF2 Antibody Picoband(r)A04692-APC
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHSF2
  • SizePrice
Figure 1. Western blot analysis of HSF2 using anti-HSF2 antibody (A04692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # A04692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-HSF2 Antibody Picoband(r)A04692-BIOTIN
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHSF2
  • SizePrice
Figure 1. Western blot analysis of HSF2 using anti-HSF2 antibody (A04692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # A04692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-HSF2 Antibody Picoband(r)A04692-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHSF2
  • SizePrice
Figure 1. Western blot analysis of HSF2 using anti-HSF2 antibody (A04692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # A04692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-HSF2 Antibody Picoband(r)A04692-CY3
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHSF2
  • SizePrice
Figure 1. Western blot analysis of HSF2 using anti-HSF2 antibody (A04692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # A04692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-HSF2 Antibody Picoband(r)A04692-DYLIGHT488
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHSF2
  • SizePrice
Figure 1. Western blot analysis of HSF2 using anti-HSF2 antibody (A04692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # A04692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-HSF2 Antibody Picoband(r)A04692-DYLIGHT550
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHSF2
  • SizePrice
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