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Western blot analysis of TMS1 expression in U937 cell lysate (M00362). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCARD monoclonal antibody (Catalog # M00362) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCARD
Product group Antibodies
Boster Bio
Anti-TMS1 Rabbit Monoclonal AntibodyM00362
IHC analysis of Elafin using anti-Elafin antibody (M00364). Elafin was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Elafin Antibody (M00364) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Elafin/Skalp Antibody (monoclonal, C7)M00364
Western blot analysis of PDGFR expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
Anti-PDGFR alpha Rabbit Monoclonal AntibodyM00366
Western blot of i(n)NOS in mouse brain crude lysate (50 μg of protein loaded).
Product group Antibodies
Boster Bio
Anti-iNOS AntibodyM00368-2
Western blot analysis of iNOS expression in Human fetal brain lysate.
Product group Antibodies
Boster Bio
Anti-iNOS Rabbit Monoclonal AntibodyM00368
Western blot analysis of Dopamine Transporter expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-Dopamine Transporter Rabbit Monoclonal AntibodyM00369
Western blot analysis of ATF4 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
Product group Antibodies
Boster Bio
Anti-ATF4 Rabbit Monoclonal AntibodyM00371
Immunohistochemical analysis of paraffin-embedded mouse colon, using MCM2 Antibody(M00374) MCM2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MCM2 Antibody (M00374)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-MCM2 Rabbit Monoclonal AntibodyM00374
Western blot analysis of TLR7 expression in Raji cell lysate (M00376). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 monoclonal antibody (Catalog # M00376) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7
Product group Antibodies
Boster Bio
Anti-TLR7 Rabbit Monoclonal AntibodyM00376
Immunohistochemical analysis of paraffin-embedded human colon, using CD46 Antibody.
Product group Antibodies
Boster Bio
Anti-CD46 Rabbit Monoclonal AntibodyM00377-1
Western blot analysis of EGF expression in human urine sample lysate (M00378). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF monoclonal antibody (Catalog # M00378) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGF
Product group Antibodies
Boster Bio
Anti-EGF Rabbit Monoclonal AntibodyM00378
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using Survivin Antibody(M00379-1) BIRC5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BIRC5 Antibody (M00379-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Survivin Rabbit Monoclonal AntibodyM00379-1
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