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Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human FGF2 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17KD. The expected band size for FGF2 is at 17KD.
Product group Antibodies
Boster Bio
Anti-FGF2 AntibodyA00121-1
Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate, Lane 2: mouse ovary tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17KD. The expected band size for FGF2 is at 17KD.
Product group Antibodies
Boster Bio
Anti-FGF2 AntibodyA00121-2
Western blot analysis of TLR2 using anti-TLR2 antibody (A00131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR2 antigen affinity purified polyclonal antibody (Catalog # A00131) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR2 at approximately 89KD. The expected band size for TLR2 is at 89KD.
Product group Antibodies
Boster Bio
Anti-TLR2 AntibodyA00131
IHC analysis of CD14 using anti-CD14 antibody (A00137). CD14 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD14 Antibody (A00137) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CD14 AntibodyA00137
Flow Cytometry analysis of BRL cells using anti-Bcl6 antibody (A00142-1). Overlay histogram showing BRL cells stained with A00142-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl6 Antibody (A00142-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Product group Antibodies
Boster Bio
Anti-BCL6 AntibodyA00142-1
Flow Cytometry analysis of HL-60 cells using anti-CD11b antibody (A00144-1). Overlay histogram showing HL-60 cells stained with A00144-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11b Antibody (A00144-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Product group Antibodies
Boster Bio
Anti-CD11b/ITGAM AntibodyA00144-1
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human CD11b DyLight 488 conjugated AntibodyA00144-Dyl488
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human CD11b DyLight 550 conjugated AntibodyA00144-Dyl550
Western blot analysis of CD11b using anti-CD11b antibody (A00144).
Product group Antibodies
Boster Bio
Anti-CD11b Picoband AntibodyA00144
Western blot analysis of Angiogenin/ANG using anti-Angiogenin/ANG antibody (A00146).
Product group Antibodies
Boster Bio
Anti-Angiogenin/ANG Picoband AntibodyA00146
Western blot analysis of Nanog using anti-Nanog antibody (A00153-3).
Product group Antibodies
Boster Bio
Anti-Nanog Picoband AntibodyA00153-3
Western blot analysis of CD19 using anti-CD19 antibody (A00154-1).
Product group Antibodies
Boster Bio
Anti-CD19 AntibodyA00154-1
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