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Western blot analysis of Survivin expression in Neuro-2a cell lysate (M00379-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Birc5 monoclonal antibody (Catalog # M00379-2) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Birc5
Product group Antibodies
Boster Bio
Anti-Survivin Rabbit Monoclonal AntibodyM00379-2
Western blot analysis of S100A9 expression in human tonsil lysate.
Product group Antibodies
Boster Bio
Anti-S100A9 Rabbit Monoclonal AntibodyM00380-1
Western blot analysis of CEBP alpha expression in U937 cell lysate (M00386). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPA monoclonal antibody (Catalog # M00386) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBPA
Product group Antibodies
Boster Bio
Anti-CEBP alpha Rabbit Monoclonal AntibodyM00386
Western blot analysis of ERCC1 expression in HeLa cell lysate (M00388). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC1 monoclonal antibody (Catalog # M00388) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERCC1
Product group Antibodies
Boster Bio
Anti-ERCC1 Rabbit Monoclonal AntibodyM00388
Western blot analysis of HNF-4-alpha expression in A549 cell lysate (M00389). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF4A monoclonal antibody (Catalog # M00389) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-HNF-4-alpha Rabbit Monoclonal AntibodyM00389
Immunohistochemical analysis of paraffin-embedded human spleen, using CCR7 Antibody.
Product group Antibodies
Boster Bio
Anti-CCR7 Rabbit Monoclonal AntibodyM00390
Western blot analysis of Retinoic Acid Receptor alpha expression in MCF-7 cell lysate (M00392). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARA monoclonal antibody (Catalog # M00392) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RARA
Product group Antibodies
Boster Bio
Anti-Retinoic Acid Receptor alpha Rabbit Monoclonal AntibodyM00392
Western blot analysis of Interferon gamma expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Interferon gamma Rabbit Monoclonal AntibodyM00393-1
Sandwich ELISA - Recombinant rat Interferon gamma protein standard curve.
Product group Antibodies
Boster Bio
Anti-Interferon gamma Antibody (monoclonal, 8E9)M00393-2
Western blot analysis of GST3 / GST pi expression in PC3 cell lysate.
Product group Antibodies
Boster Bio
Anti-GST3 / GSTP1 Monoclonal AntibodyM00394-1
Western blot analysis of GST expression in GST recombinant protein.
Product group Antibodies
Boster Bio
Anti-GST Rabbit Monoclonal AntibodyM00394
Western blot analysis of 53BP1 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-53BP1 Rabbit Monoclonal AntibodyM00397
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