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IHC analysis of Caspase-2 using anti-Caspase-2 antibody (A02384-1). Caspase-2 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caspase-2 Antibody (A02384-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase-2/CASP2 AntibodyA02384-1
Western blot analysis of Caspase-6 using anti-Caspase-6 antibody (A02631).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase-6 AntibodyA02631
IHC analysis of Glycerol kinase using anti-Glycerol kinase antibody (A02884-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Glycerol kinase/GK AntibodyA02884-1
IHC analysis of Caspase 4 using anti-Caspase 4 antibody (A02941-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase 4/CASP4 AntibodyA02941-1
Western blot analysis of Caspase 4 using anti-Caspase 4 antibody (A02941-2).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase 4/Casp4 AntibodyA02941-2
Western blot analysis of Ribonuclease 3 using anti-Ribonuclease 3 antibody (A03115-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ribonuclease 3 antigen affinity purified polyclonal antibody (Catalog # A03115-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ribonuclease 3 at approximately 18KD. The expected band size for Ribonuclease 3 is at 18KD.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Ribonuclease 3/RNASE3 AntibodyA03115-1
IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1). Creatine Kinase MM was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Creatine Kinase MM Antibody (A03452-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Creatine Kinase MM/CKM AntibodyA03452-1
IHC analysis of HAS1 using anti-HAS1 antibody (A04784-1). HAS1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-HAS1 Antibody (A04784-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Hyaluronan synthase 1/HAS1 AntibodyA04784-1
Western blot analysis of Carboxypeptidase A/CPA1 using anti-Carboxypeptidase A/CPA1 antibody (A05985-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Carboxypeptidase A/CPA1 AntibodyA05985-1
Western blot analysis of PPA1 using anti-PPA1 antibody (A07485-1).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Pyrophosphatase 1/PPA1 AntibodyA07485-1
IHC analysis of Amylase using anti-Amylase antibody (A12967).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Alpha Amylase 1 AntibodyA12967
Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-2).
Product group Antibodies
Boster Bio
Anti-TNF alpha Picoband AntibodyA00002-2
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