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Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVWF
  • SizePrice
Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVWF
  • SizePrice
Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVWF
  • SizePrice
Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVWF
  • SizePrice
Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVWF
  • SizePrice
Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVWF
  • SizePrice
Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVWF
  • SizePrice
Figure 1. IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVWF
  • SizePrice
Figure 1. Immunohistochemistry validation of VWF using Anti-von Willebrand factor VWF Antibody (A00270). Immunohistochemistry (IHC) analyzes of VWF pAb in paraffin-embedded human colon carcinoma tissue at 1:50
Product group Antibodies
Boster Bio
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetVWF
  • SizePrice
Figure 1. Western blot analysis of HEBP1 using anti-HEBP1 antibody (A00271-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 4: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEBP1 antigen affinity purified polyclonal antibody (Catalog # A00271-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEBP1 at approximately 21 kDa. The expected band size for HEBP1 is at 21 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHEBP1
  • SizePrice
Figure 1. Western blot analysis of HEBP1 using anti-HEBP1 antibody (A00271-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 4: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEBP1 antigen affinity purified polyclonal antibody (Catalog # A00271-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEBP1 at approximately 21 kDa. The expected band size for HEBP1 is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-HEBP1 Antibody Picoband(r)A00271-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHEBP1
  • SizePrice
Figure 1. Western blot analysis of HEBP1 using anti-HEBP1 antibody (A00271-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 4: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEBP1 antigen affinity purified polyclonal antibody (Catalog # A00271-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEBP1 at approximately 21 kDa. The expected band size for HEBP1 is at 21 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHEBP1
  • SizePrice

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