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All lanes : Anti-CD3Z Antibody (C-term) at 1:4000-1:8000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: Mouse thymus lysate Lysates/proteins at 20 microg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product group Antibodies
Boster Bio
Anti-CD3Z Antibody (C-term)A02421
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Porcine
TargetCD247
  • SizePrice
All lanes : Anti-CD3Z Antibody (C-term) at 1:4000-1:8000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: Mouse thymus lysate Lysates/proteins at 20 microg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product group Antibodies
Boster Bio
Anti-CD3Z Antibody (C-term)A02421
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Porcine
TargetCD247
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Phospho-CD3 zeta (Y142) CD247 AntibodyA02421Y142
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetCD247
  • SizePrice
ICC staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product group Antibodies
Boster Bio
Anti-Myosin-11 MYH11 AntibodyA02422-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetMYH11
  • SizePrice
Western blot analysis of extracts of various cell lines, using MYH11 antibody.
Product group Antibodies
Boster Bio
Anti-Myosin-11 MYH11 AntibodyA02422
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMYH11
  • SizePrice
Formalin-fixed, paraffin-embedded section of human colon stained for Bag-1 using primary antibody dilution ratio of 1:2000. Hematoxylin-eosin counterstain.
Product group Antibodies
Boster Bio
Anti-Bag-1 AntibodyA02423-1
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetBAG1
  • SizePrice
Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (A02423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # A02423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag1 at approximately 33, 46, 56 kDa. The expected band size for Bag1 is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Bag1 Antibody Picoband(r)A02423-2-10UG
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBAG1
  • SizePrice
Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (A02423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # A02423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag1 at approximately 33, 46, 56 kDa. The expected band size for Bag1 is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Bag1 Antibody Picoband(r)A02423-2-APC
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBAG1
  • SizePrice
Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (A02423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # A02423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag1 at approximately 33, 46, 56 kDa. The expected band size for Bag1 is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Bag1 Antibody Picoband(r)A02423-2-BIOTIN
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBAG1
  • SizePrice
Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (A02423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # A02423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag1 at approximately 33, 46, 56 kDa. The expected band size for Bag1 is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Bag1 Antibody Picoband(r)A02423-2-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBAG1
  • SizePrice
Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (A02423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # A02423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag1 at approximately 33, 46, 56 kDa. The expected band size for Bag1 is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Bag1 Antibody Picoband(r)A02423-2-CY3
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBAG1
  • SizePrice
Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (A02423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # A02423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag1 at approximately 33, 46, 56 kDa. The expected band size for Bag1 is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-Bag1 Antibody Picoband(r)A02423-2-DYLIGHT488
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBAG1
  • SizePrice
45678

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