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Antibody sensitivity: MBP was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with MBP antibody (6E4) Cat.-No AM00088PU-N (0.5 g/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: 100ng MBP Lane 2: 50ng Lan
Product group Antibodies
OriGene
malE Mouse Monoclonal Antibody [Clone ID: 6E4]AM00088PU-N
ApplicationsWestern Blot, ELISA
ReactivityBacteria
TargetmalE
  • SizePrice
Detection of endogenous MEK1 Whole cell lysates of serum starved tumor cells (20000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 10B1 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3
Product group Antibodies
OriGene
MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10B1]AM00089PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetMAP2K1
  • SizePrice
Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab MEK1/2-7E10 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E10]AM00090PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetMAP2K1
  • SizePrice
Detection of endogenous MEK1/2 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MEK1/2-9G3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3
Product group Antibodies
OriGene
MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9G3]AM00091PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetMAP2K1
  • SizePrice
Detection of endogenous MEK2 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MEK2-8E8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
MEK2 (MAP2K2) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8E8]AM00092PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetMAP2K2
  • SizePrice
Detection of endogenous MKK3 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The mmunoblot was probed with mab MKK3-3F5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
MEK3 (MAP2K3) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5F7]AM00094PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse
TargetMAP2K3
  • SizePrice
Detection of endogenous MKK5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK5-14B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
MEK5 (MAP2K5) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14B5]AM00095PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetMAP2K5
  • SizePrice
Detection of endogenous MKK7: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK7-10F7 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
MEK7 (MAP2K7) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10F7]AM00096PU-N
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetMAP2K7
  • SizePrice
Detection of endogenous Mxi / SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Mxi-2F2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mab Mxi-2F2 recognizes SAPK2a/p38 at 38 kDa and its splice variant Mxi2 at 30 kDa. lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA231; lane 7: T47D
Product group Antibodies
OriGene
p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2F2]AM00097PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetMAPK14
  • SizePrice
Immunoblot Analysis: Whole cell lysates of untreated (Lane 1) and pervanadate-treated (Lane 2) bend 3 cells were separated by SDS-PAGE and blotted to a PVDF membrane. The immunoblot was probed with mab eNOS Antibody (Clone 15E2) at 1 ug/ml for 1h at 15-22°C and developed by ECL (exposure time: 3min). Mab eNOS-15E2 recognizes eNOS pSer1177 at 140 kDa.
Product group Antibodies
OriGene
eNOS (NOS3) pSer1177 Mouse Monoclonal Antibody [Clone ID: 15E2]AM00098PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetNOS3
  • SizePrice
Immunoblot Analysis Nitrotyrosine-modified BSA (NOY-BSA) probed with mab NOY-7A5 at 1 g/ml.
Product group Antibodies
OriGene
Nitrotyrosine Mouse Monoclonal Antibody [Clone ID: 7A5]AM00099PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
  • SizePrice
Detection of activated p53 Recombinant C-terminal fragment of p53 was incubated with Casein Kinase II in the absence (1) or presence (2) of ATP. Proteins were separarted by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Monoclonal p53 antibody (clone 9F4) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
p53 (TP53) pSer392 Mouse Monoclonal Antibody [Clone ID: 9F4]AM00101PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetTP53
  • SizePrice
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