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IHC-P analysis of human liver tissue using GTX45599 TMPRSS6 antibody at 5microg/ml.
IHC-P analysis of human liver tissue using GTX45599 TMPRSS6 antibody at 5microg/ml.
IHC-P analysis of human liver tissue using GTX45599 TMPRSS6 antibody at 5microg/ml.

Matriptase 2 antibody, N-term

GTX45599
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTMPRSS6
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Overview

  • Supplier
    GeneTex
  • Product Name
    Matriptase 2 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. IHC-P: 2-10 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID164656
  • Target name
    TMPRSS6
  • Target description
    transmembrane serine protease 6
  • Target synonyms
    IRIDA; matriptase-2; membrane-bound mosaic serine proteinase matriptase-2; MT2; transmembrane protease serine 6; transmembrane protease, serine 6; type II transmembrane serine protease 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IU80
  • Protein Name
    Transmembrane protease serine 6
  • Scientific Description
    The protein encoded by this gene is a type II transmembrane serine proteinase that is found attached to the cell surface. The encoded protein may be involved in matrix remodeling processes in the liver. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Matriptase 2/TMPRSS6 using anti-Matriptase 2/TMPRSS6 antibody (A02093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Matriptase 2/TMPRSS6 antigen affinity purified polyclonal antibody (Catalog # A02093-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Matriptase 2/TMPRSS6 at approximately 90,95kDa. The expected band size for Matriptase 2/TMPRSS6 is at 90 kDa.
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