WB on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with the ab at 1:2,000 in TBS-Tween with 5% milk. B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An ab against the TY1 tag or the ab against MBD3 (1:2,000 in TBS-Tween & 5% milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively).