Mca-SEVNLDAEFK(Dnp) [1802078-31-4]

TargetMol Chemicals

Mca-SEVNLDAEFK(Dnp) [1802078-31-4]

16

Research Use Only

C68H88N14O27

1533.5

Mca-SEVNLDAEFK(Dnp) is a peptide FRET substrate for Beta-secretase 1 (BACE-1), featuring the Swedish Lys-Met/Asn-Leu mutation at the beta-secretase cleavage site of the amyloid precursor protein (APP). Cleavage at the -Leu-Asp- site of Mca-SEVNLDAEFK(Dnp) releases the highly fluorescent 7-methoxycoumarin (Mca) fragment, which is freed from the quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher, leading to a significantly increased fluorescence intensity that is easily detectable.

C(C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CCCCNC2=C(N(=O)=O)C=C(N(=O)=O)C=C2)C(O)=O)=O)=O)CCC(O)=O)=O)C)=O)CC(O)=O)=O)CC(C)C)=O)CC(N)=O)=O)C(C)C)=O)CCC(O)=O)=O)CO)=O)C=3C=4C(=CC(OC)=CC4)OC(=O)C3

-20°C

12352200