Anti-MDM2 pS185 (Rabbit) to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse MDM2 present in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approxi-mately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Anti-MDM2 pS185 (Rabbit) to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse MDM2 present in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approxi-mately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Mdm2 Rabbit Polyclonal Antibody
TA319465
Overview
- SupplierOriGene
- Product NameMdm2 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID17246
- Target nameMdm2
- Target descriptiontransformed mouse 3T3 cell double minute 2
- Target synonyms1700007J15Rik; AA415488; double minute 2 protein; E3 ubiquitin-protein ligase Mdm2; Mdm-2; oncoprotein Mdm2; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2
- HostRabbit
- IsotypeIgG
- Protein IDP23804
- Protein NameE3 ubiquitin-protein ligase Mdm2
- Scientific DescriptionRabbit polyclonal MDM2 phospho S185 antibody (Phospho-specific)
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203